Wang Q P, Escudier E, Roudot-Thoraval F, Abd-Al Samad I, Peynegre R, Coste A
Service d'ORL et Chirurgie Cervico-Faciale, Hôpitaux Intercommunal et Henri Mondor de Créteil, Université Paris XII, France.
Laryngoscope. 1997 Jul;107(7):926-31. doi: 10.1097/00005537-199707000-00018.
Myofibroblasts that express alpha-smooth muscle actin (alpha-SMA) are detected in many chronic inflammatory diseases. Transforming growth factor-beta (TGF-beta) is a potent inducer of myofibroblast accumulation in tissues. In this study, scattered myofibroblasts and TGF-beta were quantified and localized in nasal polyps (NPs) and normal nasal mucosa (NM). NPs were sampled in 16 patients during ethmoidectomy and NM was obtained from 10 control subjects during rhinoplasty. alpha-SMA and TGF-beta were detected using immunohistochemistry and the numbers of labeled cells were quantified (alpha-SMA and TGF-beta indices) and compared between NPs and NM. In eight NPs, in which the pedicle was preserved, alpha-SMA and TGF-beta were evaluated and compared in the pedicle, central, and tip areas. Finally, TGF-beta expression was compared between low (zone 1), moderate (zone 2), and high (zone 3) zones of alpha-SMA positivity. alpha-SMA and TGF-beta indices were significantly higher in NPs than in NM. In the eight selected NPs, alpha-SMA-positive cells were significantly more abundant in the pedicle than in the central and tip areas, whereas TGF-beta-positive cells were significantly more numerous in the pedicle than in the tip area. The number of TGF-beta-positive cells was significantly higher in zone 3 than in zone 1 of alpha-SMA positivity. Myofibroblasts, which are abundant in NPs but rare in NM, could be involved in the growth of NPs by inducing extracellular matrix accumulation. The local development of myofibroblasts in NPs could be controlled by TGF-beta, locally produced by inflammatory cells.
在许多慢性炎症性疾病中都能检测到表达α-平滑肌肌动蛋白（α-SMA）的肌成纤维细胞。转化生长因子-β（TGF-β）是组织中肌成纤维细胞积聚的强效诱导剂。在本研究中，对鼻息肉（NP）和正常鼻黏膜（NM）中的散在肌成纤维细胞和TGF-β进行了定量和定位。在16例患者行筛窦切除术时采集NP，在10例对照受试者行鼻成形术时获取NM。采用免疫组织化学法检测α-SMA和TGF-β，并对标记细胞数量进行定量（α-SMA和TGF-β指数），比较NP和NM之间的差异。在8个保留蒂部的NP中，对蒂部、中央和尖端区域的α-SMA和TGF-β进行评估和比较。最后，比较α-SMA阳性的低（1区）、中（2区）、高（3区）区域之间的TGF-β表达。NP中的α-SMA和TGF-β指数显著高于NM。在8个选定的NP中，蒂部的α-SMA阳性细胞明显多于中央和尖端区域，而蒂部的TGF-β阳性细胞明显多于尖端区域。α-SMA阳性的3区TGF-β阳性细胞数量明显高于1区。肌成纤维细胞在NP中丰富而在NM中罕见，可能通过诱导细胞外基质积聚参与NP的生长。NP中肌成纤维细胞的局部发育可能受炎症细胞局部产生的TGF-β控制。
