Coste A, Lefaucheur J P, Wang Q P, Lesprit E, Poron F, Peynegre R, Escudier E
Department of Oto-Rhino-Laryngology and Head and Neck Surgery, Hôpital Intercommunal and Centre Hospitalo-Universitaire Henri Mondor de Créteil, Institut National de la Santé et de la Recherche Médicale, Unite U 296, Faculty o.
Arch Otolaryngol Head Neck Surg. 1998 Dec;124(12):1361-6. doi: 10.1001/archotol.124.12.1361.
To determine the expression and the potential role of transforming growth factor beta (TGF-beta) in nasal polyposis.
Comparison of TGF-beta expression between normal and inflammatory nasal mucosa and polyps; in inflammatory nasal polyps, characterization of the TGF-beta isoforms expression and their potential location in macrophages and eosinophils.
Patients and samples were selected at the Hôpital Intercommunal, Créteil, France, and immunohistochemistry and immunoblots were performed at the Institut National de la Sante et de la Recherche Medicale U296 (Universite Paris XII, France).
Nasal polyps and nasal mucosa were sampled in 21 patients during ethmoidectomy, and muscosa was sampled in 6 healthy patients during rhinoplasty.
Immunohistochemistry and Western blot analysis were performed using specific antibodies to TGF-beta1-3, TGF-beta1, TGF-beta2, and TGF-beta3 isoforms. Double labeling was also performed using anti-TGF-beta1 antibody together with macrophages or eosinophil-specific antibodies.
The expression of TGF-beta(1-3) was significantly higher in inflammatory nasal polyps than in inflammatory nasal mucosa and higher in inflammatory nasal mucosa than in nasal mucosa from healthy patients. Transforming growth factor beta1 was the main isoform detected in inflammatory nasal polyps, and it was present in numerous macrophages and in some eosinophils.
Transforming growth factor beta, mainly TGF-beta1, is strongly expressed in inflammatory nasal mucosa, where it could be produced by macrophages and eosinophils. Transforming growth factor beta could induce epithelium and connective tissue modifications and therefore be involved in the pathogenesis of nasal polyposis.
确定转化生长因子β（TGF-β）在鼻息肉中的表达及潜在作用。
比较正常鼻黏膜、炎性鼻黏膜和息肉中TGF-β的表达；在炎性鼻息肉中，鉴定TGF-β亚型的表达及其在巨噬细胞和嗜酸性粒细胞中的潜在定位。
患者和样本选自法国克雷泰伊市公共医院，免疫组织化学和免疫印迹检测在法国国家健康与医学研究院U296（法国巴黎第十二大学）进行。
21例患者在筛窦切除术时采集鼻息肉和鼻黏膜样本，6例健康患者在鼻成形术时采集鼻黏膜样本。
使用针对TGF-β1 - 3、TGF-β1、TGF-β2和TGF-β3亚型的特异性抗体进行免疫组织化学和蛋白质印迹分析。还使用抗TGF-β1抗体与巨噬细胞或嗜酸性粒细胞特异性抗体进行双重标记。
炎性鼻息肉中TGF-β（1 - 3）的表达显著高于炎性鼻黏膜，炎性鼻黏膜中的表达高于健康患者的鼻黏膜。转化生长因子β1是炎性鼻息肉中检测到的主要亚型，存在于大量巨噬细胞和一些嗜酸性粒细胞中。
转化生长因子β，主要是TGF-β1，在炎性鼻黏膜中强烈表达，可能由巨噬细胞和嗜酸性粒细胞产生。转化生长因子β可诱导上皮和结缔组织改变，因此参与鼻息肉的发病机制。
