Doumanova L, Alexandrov M
Department of Virology, Central Veterinary Research Institute, Sofia, Bulgaria.
Acta Virol. 1997 Apr;41(2):111-4.
Various electron microscopic (EM) and immunoelectron microscopic (IEM) techniques were used to demonstrate and identify the Newcastle disease virus (NDV) infection. By IEM, the number of virions in native allantoic fluids was increased 50-100 times in comparison with direct EM. The immunogold staining showed that a number of immunogold particles were specifically bound to the antigen determinants located on the virion surface and these results were much easier to interpret. The obtained results showed that the EM and IEM can be successfully employed for a precise and rapid detection of NDV as well as for identification of this infection among other viral or bacterial infections.
采用多种电子显微镜（EM）和免疫电子显微镜（IEM）技术来证实和鉴定新城疫病毒（NDV）感染。通过IEM发现，与直接EM相比，天然尿囊液中的病毒粒子数量增加了50 - 100倍。免疫金染色显示，许多免疫金颗粒特异性结合到病毒粒子表面的抗原决定簇上，这些结果更易于解读。所得结果表明，EM和IEM可成功用于精确、快速检测NDV以及在其他病毒或细菌感染中鉴定这种感染。
