Hele P, Hebert L
Biochim Biophys Acta. 1977 Dec 2;479(3):311-21. doi: 10.1016/0005-2787(77)90113-7.
(1) Postmicrosomal particles were isolated from the cytosol of lactating rat mammary gland. These particles contained up to 40% of the cytosol protein, and about 40% of the total aminoacyl-tRNA synthetase activity of the cytosol. (2) Sucrose density gradient centrifugation of these postmicrosomal particles showed that the activity of seventeen synthetases was distributed between one or other of two major gradient regions, corresponding to values of 20--28 S and 5--7 S. Arginyl and cysteinyl tRNA synthetases showed peaks in both major regions. (3) A complex of eight aminoacyl-tRNA synthetases (those for lysine, leucine, isoleucine, methionine, glutamine, arginine, cysteine and valine) of molecular weight 3 - 10(6) was isolated from the postmicrosomal particles by gel filtration and chromatography on DEAE-cellulose.
(1) 从泌乳大鼠乳腺的胞质溶胶中分离出微粒体后颗粒。这些颗粒含有高达40%的胞质溶胶蛋白，以及胞质溶胶中约40%的总氨酰-tRNA合成酶活性。(2) 对这些微粒体后颗粒进行蔗糖密度梯度离心，结果显示17种合成酶的活性分布在两个主要梯度区域中的一个或另一个区域，分别对应于20 - 28 S和5 - 7 S的值。精氨酰和半胱氨酰tRNA合成酶在两个主要区域均出现峰值。(3) 通过凝胶过滤和DEAE-纤维素柱色谱从微粒体后颗粒中分离出一种分子量为3 - 10(6)的由8种氨酰-tRNA合成酶（赖氨酸、亮氨酸、异亮氨酸、蛋氨酸、谷氨酰胺、精氨酸、半胱氨酸和缬氨酸的合成酶）组成的复合物。
