Sopp P, Howard C J
Institute for Animal Health, Compton Laboratory, Newbury, Berkshire, UK.
Vet Immunol Immunopathol. 1997 May;56(1-2):11-25. doi: 10.1016/s0165-2427(96)05731-5.
Thirty-seven subpanels of monoclonal antibodies (mAbs) included within the Vth International Workshop on Human Leucocyte Differentiation Antigens (Vth Workshop) were assayed for reactivity with bovine peripheral blood leucocytes. Sixty-five of the 772 mAbs (8.4%) stained bovine cells. mAbs from each of the 27 different CD groups that contained a mAb reacting with cattle were further investigated to compare the cellular expression of the antigen in cattle with that reported for the different CD antigens in humans. Two-colour immunofluorescence staining of the Vth Workshop mAbs against characterized bovine leucocyte subpopulation markers that identified monocytes, B cells, CD4, CD8 and WC1 +T cells were used for these analyses. Eighteen of the mAbs to different human CD antigens (CD11a, CD14, CD18, CD21, CD27, CD29, CD49a, CD49b, CD49d, CD49e, CD51, CD61, CD62L, CD62P, CD63, CDw78, CD98, CD100) stained bovine antigens with an almost identical cellular distribution to that reported in humans. This implies that these mAb react with the homologous cattle molecules. Nine mAbs (CD35, CD37, CD49c, CD50, CD54, CD66, CD81, CD88, CD102) stained bovine cells but the cellular distribution of the bovine antigen was different to that reported in humans implying either a different cellular distribution for these antigens in cattle or a reaction with a different molecule. The investigation has allowed the identification of several bovine homologues of human CD antigens that have not been previously defined in cattle and the cross-reacting mAbs will be valuable reagents for future investigations of bovine immunology.
在第五届人类白细胞分化抗原国际研讨会（第五届研讨会）中包含的37个单克隆抗体（mAb）亚组，被检测与牛外周血白细胞的反应性。772个mAb中的65个（8.4%）对牛细胞进行了染色。对包含与牛反应的mAb的27个不同CD组中的每一个组的mAb进行了进一步研究，以比较牛中抗原的细胞表达与人类中不同CD抗原的报道情况。使用第五届研讨会的mAb对已鉴定的牛白细胞亚群标志物（识别单核细胞、B细胞、CD4、CD8和WC1 + T细胞）进行双色免疫荧光染色，用于这些分析。针对不同人类CD抗原（CD11a、CD14、CD18、CD21、CD27、CD29、CD49a、CD49b、CD49d、CD49e、CD51、CD61、CD62L、CD62P、CD63、CDw78、CD98、CD100）的18个mAb对牛抗原进行了染色，其细胞分布与人类报道的几乎相同。这意味着这些mAb与同源的牛分子发生反应。9个mAb（CD35、CD37、CD49c、CD50、CD54、CD66、CD81、CD88、CD102）对牛细胞进行了染色，但牛抗原的细胞分布与人类报道的不同，这意味着这些抗原在牛中的细胞分布不同，或者与不同的分子发生反应。该研究已鉴定出几种以前在牛中未定义的人类CD抗原的牛同源物，这些交叉反应的mAb将是未来牛免疫学研究的有价值试剂。
