一种使用激光和横向电光调制器进行亚纳秒寿命测量的相移荧光计。
A phase-shift fluorometer using a laser and a transverse electrooptic modulator for subnanosecond lifetime measurements.
作者信息
Salmeen I, Rimai L
出版信息
Biophys J. 1977 Dec;20(3):335-42. doi: 10.1016/S0006-3495(77)85553-7.
We described a simple phase-shift fluorometer using continuous laser excitation. The laser enables the use of a transverse mode electrooptic modulator with a half-wave retardation voltage of about 200 V (in contrast to many kilovolts of longitudinal modulators) at frequencies up to 100 MHz. The modulated fluorescence signal is detected, after passing through a double monochromator, by a photomultiplier tube feeding a radio frequency (RF) tuned amplifier. THE RF phase is then determined by phase-sensitive detection using a double balanced mixer with the reference obtained from a PIN photodiode-turned amplifier combination which detects light split off from the main exciting beam. The laser and double monochromator allow the observation of modulated Raman solvent and Rayleigh scatterin, which are convenient for determining the zero reference phase.
我们描述了一种使用连续激光激发的简单相移荧光计。该激光器能够使用横向模式电光调制器，其半波延迟电压约为200V（与纵向调制器的数千伏形成对比），频率高达100MHz。调制后的荧光信号在通过双单色仪后，由一个向射频（RF）调谐放大器馈电的光电倍增管进行检测。然后，通过使用双平衡混频器进行相敏检测来确定RF相位，参考信号来自一个PIN光电二极管 - 转换放大器组合，该组合检测从主激发光束中分出的光。激光和双单色仪允许观察调制的拉曼溶剂和瑞利散射，这便于确定零参考相位。
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