Mendelson D S, Metz E N, Sagone A L
Blood. 1977 Dec;50(6):1023-30.
The role of reduced glutathione in relation to hexose monophosphate shunt activity and peroxide detoxification has been well established in human erythrocytes. Less is known about the content of reduced glutathione in phagocytic leukocytes and the changes that occur during functional activity. We have measured the reduced sulfhydryl content of normal resting human granulocytes and of cells isolated from a patient with chronic granulomatous disease. Normal cells and those from the patient with chronic granulomatous disease contained similar concentrations of reduced sulfhydryls. Stimulation of a phagocytic response by incubation with opsonized zymosan particles resulted in prompt and nearly complete depletion of intracellular glutathione from normal granulocytes. This fall in reduced glutathione concentration was dependent on the phagocytic load. Exposure of chronic granulomatous disease granulocytes to a similar phagocytic load resulted in a slower and less complete fall in reduced glutathione. In normal cells, those from the chronic granulomatous disease patient, and those from an obligate carrier of the disease, the decrement in reduced glutathione during phagocytosis was correlated with oxidation of 14C-1-glucose and 14C-formate, nitroblue tetrazolium reduction, and the chemiluminescence phenomenon.
还原型谷胱甘肽在人类红细胞中与磷酸己糖旁路活性及过氧化物解毒的关系已得到充分证实。关于吞噬性白细胞中还原型谷胱甘肽的含量以及功能活动期间发生的变化，人们了解得较少。我们测定了正常静息人类粒细胞以及从一名慢性肉芽肿病患者分离出的细胞中还原巯基的含量。正常细胞和慢性肉芽肿病患者的细胞含有相似浓度的还原巯基。用调理酵母聚糖颗粒孵育刺激吞噬反应，导致正常粒细胞内的细胞内谷胱甘肽迅速且几乎完全耗尽。还原型谷胱甘肽浓度的这种下降取决于吞噬负荷。将慢性肉芽肿病粒细胞暴露于相似的吞噬负荷下，导致还原型谷胱甘肽下降较慢且不完全。在正常细胞、慢性肉芽肿病患者的细胞以及该病的一名携带者的细胞中，吞噬过程中还原型谷胱甘肽的减少与14C-1-葡萄糖和14C-甲酸的氧化、硝基蓝四氮唑还原以及化学发光现象相关。
