Kraut E H, Segal M, Sagone A L
Inflammation. 1982 Jun;6(2):161-7. doi: 10.1007/BF00916240.
oxygen radicals have been shown to alter granulocyte function by injury to the cell membrane or cytoskeleton. We have investigated the effect of such injury on the aggregation of granulocytes upon C5a or PMA stimulation. Granulocyte aggregation was not altered in the presence of the oxygen radical scavengers SOD, catalase, mannitol, or benzoate. To test whether oxygen radicals were required for aggregation, we evaluated three patients with chronic granulomatous disease of childhood. The response of PMNs from these patients was no different from controls. Hydrocortisone, an inhibitor of both granulocyte aggregation and oxygen radical generation, was then studied to decide whether its impairment of radical production contributed to its effect on aggregation. Five times the concentration of hydrocortisone needed to inhibit radical generation was required to impair aggregation by 50%. In addition, hydrocortisone was able to impair the aggregation of the PMNs of a patient with chronic granulomatous disease. These data suggest that oxidative injury to the cell membrane or cytoskeleton does not significantly contribute to the aggregation of granulocytes. In addition, inhibitors of aggregation, such as hydrocortisone, work through mechanisms other than by scavenging radicals.
氧自由基已被证明可通过损伤细胞膜或细胞骨架来改变粒细胞功能。我们研究了这种损伤对C5a或PMA刺激下粒细胞聚集的影响。在存在氧自由基清除剂超氧化物歧化酶（SOD）、过氧化氢酶、甘露醇或苯甲酸盐的情况下，粒细胞聚集未发生改变。为了测试聚集是否需要氧自由基，我们评估了三名儿童慢性肉芽肿病患者。这些患者的中性粒细胞反应与对照组无异。然后研究了氢化可的松，它是粒细胞聚集和氧自由基生成的抑制剂，以确定其对自由基产生的抑制作用是否导致其对聚集的影响。抑制自由基生成所需氢化可的松浓度的五倍才能使聚集减少50%。此外，氢化可的松能够削弱一名慢性肉芽肿病患者中性粒细胞的聚集。这些数据表明，细胞膜或细胞骨架的氧化损伤对粒细胞聚集没有显著影响。此外，聚集抑制剂，如氢化可的松，是通过清除自由基以外的机制起作用的。
