Ogawa M, Grush O C, O'Dell R F, Hara H, MacEachern M D
Blood. 1977 Dec;50(6):1081-92.
Circulating erythropoietic precursors in normal men and patients with hemoglobinopathies were characterized in culture. Blood mononuclear cells harvested with a modification of the Ficoll-Isopaque technique were cultured in methylcellulose for 14 days. The majority of erythropoietic colonies consisted of several subcolonies assuming the morphology of erythropoietic "bursts" described in murine marrow cultures. Time course studied of colony formation from marrow and blood nucleated cells confirmed that the circulating erythropoietic precursors represented only early stages of development. Peak sedimentation velocity of the circulating precursors analyzed using a Staput apparatus averaged 5.31 mm/hr and corresponded with that of the early erythropoietic precursors in human marrow. One ml of blood yielded an average of 153 colonies in normal men and 785 colonies in patients with hemoglobinopathies. No correlation was observed between colony formation and reticulocyte indices of individual patients. Examination of the proliferative state of the erythropoietic precursors using high specific activity tritium-labeled thymidine revealed that almost none of the cells in normal men or patients with hemoglobinopathies were in the DNA synthetic phase.
对正常男性和血红蛋白病患者循环中的红细胞生成前体细胞进行了培养特性研究。采用改良的Ficoll-泛影葡胺技术采集血液单核细胞，在甲基纤维素中培养14天。大多数红细胞生成集落由几个亚集落组成，呈现出小鼠骨髓培养中所描述的红细胞生成“爆发”形态。对骨髓和血液有核细胞集落形成的时间进程研究证实，循环中的红细胞生成前体细胞仅代表发育的早期阶段。使用Staput仪器分析循环前体细胞的峰值沉降速度平均为5.31毫米/小时，与人类骨髓中早期红细胞生成前体细胞的沉降速度一致。正常男性每毫升血液平均产生153个集落，血红蛋白病患者每毫升血液平均产生785个集落。未观察到个体患者的集落形成与网织红细胞指数之间存在相关性。使用高比活度氚标记胸腺嘧啶核苷检测红细胞生成前体细胞的增殖状态，结果显示正常男性和血红蛋白病患者中几乎没有细胞处于DNA合成期。
