Castelnovo P, Albanesi C
Medicinal Chemistry Analytical Laboratory, Zambon Group, Bresso (Milan), Italy.
Electrophoresis. 1997 Jun;18(6):996-1001. doi: 10.1002/elps.1150180622.
N-Propionyl-6,7-dimethoxy-2-aminotetralin (Z12231A), a useful intermediate in the synthesis of dopaminergic agonists, was resolved into its enantiomers by high-performance capillary electrophoresis. Cyclodextrin-modified micellar electrokinetic chromatography was employed with a combination of chiral selectors both in the aqueous phase (a hydroxyalkyl-beta-cyclodextrin) and in the micellar phase (sodium taurodeoxycholate). The absolute amount of the two chiral selectors as well as their ratio were found to be the most critical parameters in order to optimize separation. A resolution factor >3.5 was obtained, allowing a high amount of solute to be loaded in order to improve the detection limit. The reproducibility of the assay was also evaluated.
N-丙酰基-6,7-二甲氧基-2-氨基四氢萘（Z12231A）是合成多巴胺能激动剂的一种有用中间体，通过高效毛细管电泳拆分为对映体。采用环糊精修饰的胶束电动色谱法，在手性选择剂的水相（羟烷基-β-环糊精）和胶束相（牛磺脱氧胆酸钠）中进行组合。为了优化分离，发现两种手性选择剂的绝对量及其比例是最关键的参数。获得了大于3.5的分离度因子，可加载大量溶质以提高检测限。还评估了该测定法的重现性。
