Yokoyama M, Ishiwata T, Aida T, Asano G
Department of Pathology, Nippon Medical School, Tokyo, Japan.
J Atheroscler Thromb. 1994;1(1):60-70. doi: 10.5551/jat1994.1.60.
During re-endothelialization after intimal denudation induced by balloon catheter in the rat aorta, re-formation of the basement membrane (BM) was examined in samples taken 15 min, 3, 7, 14, 21 and 28 days after the balloon-induced injury. Although the luminal surface of the aortic wall was covered by round-shaped regenerating endothelial cells (ECs) by 7 days after intimal denudation, no continuous BM structure was detectable until 21 days. Periodic acid-thiosemicarbazide gelatin-methenamine silver (PATSC-GMS) staining for electron microscopy and immunostaining of type IV collagen and laminin demonstrated the accumulation of BM components underneath the regenerating ECs after 14 days. The expression of type IV collagen mRNA was revealed in regenerating ECs by in situ hybridization. A continuous BM structure first appeared 21 days after intimal denudation and was almost complete by 28 days. Simultaneously, the regenerating ECs flattened and attached more closely to the BM than in earlier phases. In conclusion, we consider that the regenerating ECs produce the BM components and suggest that reorganization of the newly formed BM is important in the process of differentiation of regenerating ECs.
在大鼠主动脉球囊导管诱导内膜剥脱后的再内皮化过程中，于球囊损伤后15分钟、3天、7天、14天、21天和28天采集样本，检测基底膜（BM）的重新形成情况。尽管内膜剥脱后7天主动脉壁腔面被圆形的再生内皮细胞（ECs）覆盖，但直至21天仍未检测到连续的BM结构。用于电子显微镜检查的高碘酸-硫代半卡巴腙明胶-亚甲胺银（PATSC-GMS）染色以及IV型胶原和层粘连蛋白的免疫染色显示，14天后BM成分在再生ECs下方积累。通过原位杂交在再生ECs中检测到IV型胶原mRNA的表达。连续的BM结构在内膜剥脱后21天首次出现，到28天时几乎完全形成。同时，再生ECs变得扁平，并且比早期阶段更紧密地附着于BM。总之，我们认为再生ECs产生BM成分，并表明新形成的BM的重组在再生ECs的分化过程中很重要。
