Jenkins R E, Taylor M J, Gilvary N, Bianco A E
Liverpool School of Tropical Medicine, UK.
Parasite Immunol. 1996 Jan;18(1):29-42. doi: 10.1046/j.1365-3024.1996.d01-10.x.
A cDNA designated MOv2 was isolated from an Onchocerca volvulus library on the basis of its product's recognition by an antiserum raised against the infective stage. Immunogold electron microscopy revealed a high density of antigenic sites associated with the annulae of the L3 cuticle and with the uterine wall of the adult female: a general, low density of labelling occurred in all developmental forms. Western blotting confirmed the presence of the antigen throughout the life cycle and the existence of an immunologically cross-reactive homologue in the related filaria, Acanthocheilonema viteae. The antigen was shown to be secreted by infective larvae and adult females of A. viteae. Sequence comparisons revealed two homologues of MOv2 (Ov-20, Ov-9) which had been selected independently by other laboratories on the basis of their specific recognition by human onchocerciasis infection sera. The IgG antibody response against MOv2 in cattle experimentally infected with O. lienalis revealed the induction of a response during the prepatent period that was strongly boosted at the onset of patency. However, only a proportion of infected cattle responded with a detectable level of anti-MOv2 antibodies. The appearance of MOv2 in larval cuticle and secretions prompted us to evaluate it as a candidate molecule for prophylactic immunization. Trials performed in the A. viteae/Mongolian jird model of filariasis revealed that recombinant MOv2 induced a host-protective response, reducing worm recoveries by 36-55% following a challenge infection.
基于抗感染期抗血清对其产物的识别，从盘尾丝虫文库中分离出一个名为MOv2的cDNA。免疫金电子显微镜显示，L3角质层的环纹和成年雌性的子宫壁上存在高密度的抗原位点：在所有发育形式中都出现了普遍的低密度标记。蛋白质印迹法证实了该抗原在整个生命周期中的存在，以及在相关丝虫——旋盘尾丝虫中存在免疫交叉反应性同源物。该抗原被证明是由旋盘尾丝虫的感染性幼虫和成年雌性分泌的。序列比较揭示了MOv2的两个同源物（Ov - 20、Ov - 9），其他实验室根据人类盘尾丝虫病感染血清对它们的特异性识别独立选择了这两个同源物。在实验感染链尾丝虫的牛中，针对MOv2的IgG抗体反应显示，在潜伏期诱导了一种反应，在感染期开始时这种反应得到了强烈增强。然而，只有一部分感染牛产生了可检测水平的抗MOv2抗体。MOv2在幼虫角质层和分泌物中的出现促使我们将其评估为预防性免疫的候选分子。在旋盘尾丝虫/蒙古沙鼠丝虫病模型中进行的试验表明，重组MOv2诱导了宿主保护性反应，在攻击感染后使虫体回收率降低了36% - 55%。
