Taylor M J, Jenkins R E, Bianco A E
Liverpool School of Tropical Medicine, UK.
Parasite Immunol. 1996 May;18(5):219-25. doi: 10.1046/j.1365-3024.1996.d01-93.x.
A cDNA clone of Onchocerca volvulus, designated MOv14, and encoding 136 amino-acid residues from the C-terminus of O. volvulus tropomyosin, was evaluated as a protective immunogen in two complimentary rodent models of onchocerciasis. Vaccination of BALB/c mice with the recombinant fusion of MOv14 coupled to Maltose-Binding Protein (MBP) induced significant reductions (48-62%) in the recovery of Onchocerca lienalis microfilariae from the skin, compared to control groups immunized with MBP alone. The predominant antibody response generated to MOv14 by vaccination was of IgG1. Following a similar vaccination protocol in Mongolian jirds, two independent experiments demonstrated that 16 weeks after infection with Acanthocheilonema viteae there was a 46% reduction in the recovery of adult worms in vaccinated animals compared to control groups. Antibodies generated by vaccination recognized a product released during culture of A. viteae infective larvae which migrated at a distinct molecular mass from native tropomyosin from somatic tissues.
盘尾丝虫的一个cDNA克隆，命名为MOv14，编码盘尾丝虫原肌球蛋白C末端的136个氨基酸残基，在盘尾丝虫病的两种互补啮齿动物模型中被评估为一种保护性免疫原。用与麦芽糖结合蛋白（MBP）偶联的MOv14重组融合物对BALB/c小鼠进行疫苗接种，与仅用MBP免疫的对照组相比，从皮肤中回收的盘尾丝虫微丝蚴显著减少（48 - 62%）。疫苗接种对MOv14产生的主要抗体反应为IgG1。在蒙古沙鼠中采用类似的疫苗接种方案后，两项独立实验表明，与对照组相比，感染罗阿罗阿丝虫16周后，接种疫苗的动物体内成虫的回收率降低了46%。疫苗接种产生的抗体识别罗阿罗阿丝虫感染性幼虫培养过程中释放的一种产物，该产物迁移的分子量与体细胞组织中的天然原肌球蛋白不同。
