布氏锥虫发育调控机制：表面蛋白mRNA 3'非翻译区的一个多聚嘧啶序列影响RNA丰度和翻译。
Mechanisms of developmental regulation in Trypanosoma brucei: a polypyrimidine tract in the 3'-untranslated region of a surface protein mRNA affects RNA abundance and translation.
作者信息
Hotz H R, Hartmann C, Huober K, Hug M, Clayton C
机构信息
Zentrum für Molekulare Biologie, Universität Heidelberg, Im Neuenheimer Feld 282, D-69120 Heidelberg, Germany.
出版信息
Nucleic Acids Res. 1997 Aug 1;25(15):3017-26. doi: 10.1093/nar/25.15.3017.
Abstract
Salivarian trypanosomes are extracellular parasites of mammals that are transmitted by tsetse flies. The procyclic acidic repetitive proteins (PARPs) are the major surface glycoproteins of the form of Trypanosoma brucei that replicates in the fly. The abundance of PARP mRNA and protein is very strongly regulated, mostly at the post-transcriptional level. The 3'-untranslated regions of two PARP genes are of similar lengths, but are dissimilar in sequence apart from a 16mer stem-loop that stimulates translation and a 26mer polypyrimidine tract. Addition of either of these PARP 3'-untranslated regions immediately downstream of a reporter gene resulted in developmental regulation mimicking that of PARP. We show that the PARP 3'-UTR reduces RNA stability and translation in bloodstream forms and that the 26mer polypyrimidine tract is necessary for both effects.
摘要
涎源性锥虫是由采采蝇传播的哺乳动物细胞外寄生虫。前循环酸性重复蛋白（PARP）是在采采蝇体内复制的布氏锥虫形态的主要表面糖蛋白。PARP mRNA和蛋白质的丰度受到非常严格的调控，主要是在转录后水平。两个PARP基因的3'非翻译区长度相似，但除了一个刺激翻译的16聚体茎环和一个26聚体多嘧啶序列外，序列不同。将这些PARP 3'非翻译区中的任何一个添加到报告基因的紧下游，都会导致发育调控，模仿PARP的调控。我们表明，PARP 3'-UTR降低了血流形式中的RNA稳定性和翻译，并且26聚体多嘧啶序列对这两种效应都是必需的。
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