Leuker C E, Sonneborn A, Delbrück S, Ernst J F
Institut für Mikrobiologie, Heinrich-Heine-Universität, Düsseldorf, Germany.
Gene. 1997 Jun 19;192(2):235-40. doi: 10.1016/s0378-1119(97)00069-3.
The PCK1 gene encoding PEP carboxykinase (Pck1) of the fungal pathogen Candida albicans was isolated and sequenced. The deduced Pck1 protein has high homology to ATP-dependent Pck1 proteins in other species, especially to Pck1 of Saccharomyces cerevisiae (70% homology), but not to GTP-dependent Pck1 proteins. PCK1 transcript levels were efficiently repressed by glucose and derepressed (induced) on gluconeogenetic carbon sources. PCK1 regulation occurs on the level of transcription, as demonstrated by a fusion of the PCK1 promoter to the LAC4 reporter gene, yielding derepressed/repressed expression ratios of > 100. Homologous sequences in the PCK1 promoters of C. albicans and S. cerevisiae were identified. The PCK1 promoter may be useful to efficiently regulate expression and thereby test the function of genes in C. albicans.
对真菌病原体白色念珠菌中编码磷酸烯醇式丙酮酸羧激酶（Pck1）的PCK1基因进行了分离和测序。推导的Pck1蛋白与其他物种中依赖ATP的Pck1蛋白具有高度同源性，尤其是与酿酒酵母的Pck1（同源性为70%），但与依赖GTP的Pck1蛋白无同源性。PCK1转录水平在葡萄糖存在时被有效抑制，而在糖异生碳源上则被解除抑制（诱导）。如将PCK1启动子与LAC4报告基因融合所示，PCK1的调控发生在转录水平，产生的解除抑制/抑制表达比>100。在白色念珠菌和酿酒酵母的PCK1启动子中鉴定出同源序列。PCK1启动子可能有助于有效调控表达，从而测试白色念珠菌中基因的功能。
