Szafranski P, Smith C L, Cantor C R
Center for Advanced Biotechnology and Department of Biomedical Engineering, Boston University, MA 02215, USA.
Biochim Biophys Acta. 1997 Jun 26;1352(3):243-8. doi: 10.1016/s0167-4781(97)00059-6.
The dnaG gene coding for primase, a key enzyme in DNA replication, has been isolated from chromosomal DNA of the soil bacterium Pseudomonas putida. It maps within the putative MMS operon, between the rpsU and rpoD genes. Comparison of the deduced amino acid sequence of P. putida DnaG with sequences of other known bacterial primases reveals the presence of a possible regulatory region which would be unique to pseudomonads. The analysis of nucleotide sequence suggests that stable folding of the dnaG mRNA may significantly contribute to the low level of its expression within a cell.
编码引发酶（DNA复制中的关键酶）的dnaG基因已从土壤细菌恶臭假单胞菌的染色体DNA中分离出来。它定位于假定的MMS操纵子内，在rpsU和rpoD基因之间。将恶臭假单胞菌DnaG的推导氨基酸序列与其他已知细菌引发酶的序列进行比较，发现存在一个可能的调控区域，这在假单胞菌中是独特的。核苷酸序列分析表明，dnaG mRNA的稳定折叠可能对其在细胞内的低表达水平有显著贡献。
