Hankard G F, Matarazzo P, Duong J P, Mougenot J F, Navarro J, Cézard J P, Peuchmaur M
Service d'Anatomie Pathologique et de Gastroenterologie Pédiatrique, Hôpital Robert Debré, Paris, France.
J Pediatr Gastroenterol Nutr. 1997 Jul;25(1):79-83. doi: 10.1097/00005176-199707000-00013.
Although a high level of intraepithelial lymphocytes (IELs) has been demonstrated in intestinal biopsies from children with cow's milk protein intolerance (CMPI), the properties of IELs in food-sensitive enteropathies remain unclear. In the present study, we analyzed the cytotoxic potential of IELs in CMPI, using a monoclonal antibody directed against the cytotoxic granule-associated protein TIA1.
The study included 18 duodenal biopsies from 10 children previously diagnosed with CMPI and on a cow's milk-free diet of various duration. Six normal duodenal biopsies from children free of food intolerance served as controls. Immunostaining of formalin-fixed tissues was used to determine in the intraepithelial compartment (1) the number of TIA1-expressing cells per 100 epithelial cells, (2) the number of IELs per 100 epithelial cells, (3) the ratio of TIA1-expressing IELs (TIA1/IEL ratio).
In CMPI, the number of IELs and TIA1-positive cells, as well as the TIA1/IEL ratio was significantly increased compared with controls. Moreover, a negative correlation between the TIA1/IEL ratio and the duration of the diet was observed.
These results suggest that the recruitment of IELs with cytotoxic potential is increased in CMPI, and that IEL-mediated cytotoxicity could be involved in the pathogenesis of the disease.
尽管已证实在患有牛奶蛋白不耐受（CMPI）的儿童肠道活检组织中上皮内淋巴细胞（IELs）水平较高，但食物敏感型肠病中IELs的特性仍不清楚。在本研究中，我们使用一种针对细胞毒性颗粒相关蛋白TIA1的单克隆抗体，分析了CMPI中IELs的细胞毒性潜能。
本研究纳入了10名先前诊断为CMPI且已采用不同时长无牛奶饮食的儿童的18份十二指肠活检组织。6份来自无食物不耐受儿童的正常十二指肠活检组织作为对照。采用福尔马林固定组织免疫染色法，在上皮内区域测定：（1）每100个上皮细胞中表达TIA1的细胞数量；（2）每100个上皮细胞中IELs的数量；（3）表达TIA1的IELs的比例（TIA1/IEL比例）。
与对照组相比，CMPI中IELs数量、TIA1阳性细胞数量以及TIA1/IEL比例均显著增加。此外，还观察到TIA1/IEL比例与饮食时长之间呈负相关。
这些结果表明，CMPI中具有细胞毒性潜能的IELs募集增加，且IEL介导的细胞毒性可能参与了该疾病的发病机制。
