Xu J N, Qu F Y
Department of Parasitology, Second Military Medical University, Shanghai, People's Republic of China.
Med Vet Entomol. 1997 Apr;11(2):134-8. doi: 10.1111/j.1365-2915.1997.tb00302.x.
Species A and D of the Anopheles dirus complex were found in China. Ribosomal DNA second internal transcribed spacers (ITS2) of both species A and D were sequenced and found to be 716 and 710 base-pairs in length, respectively, with 69% GC content. No evidence of intraspecific variation was detected in the ITS2 sequence of species A, whereas the sequence of species D showed variation at one position in the ITS2. A large number of simple repeat motifs were dispersed throughout the ITS2 sequences. The level of interspecific difference was 5.4% of the nucleotide sequences. Some of the interspecific differences were located in regions with subrepeat structure.
