Beebe N W, Ellis J T, Cooper R D, Saul A
Molecular Parasitology Unit, Department of Cell and Molecular Biology, University of Technology, Sydney, Australia.
Insect Mol Biol. 1999 Aug;8(3):381-90. doi: 10.1046/j.1365-2583.1999.83127.x.
The internal transcribed spacer 2 (ITS2) from the ribosomal DNA was sequenced and characterized for ten cryptic species in the Anopheles punctulatus group, the members of which are major vectors of malaria and filariasis in the south-west Pacific. The length of the ITS2 ranged from 549 bp to 565 bp and displayed levels of sequence variation ranging from 2.3% to 24.3% due mainly to indels of simple sequences. The GC content varied from 61.3% to 70.9%. These values were higher than those found in other cryptic species of mosquitoes and comparable only to members of the An. dirus complex suggesting a possible link between this group of Asian mosquitoes and the An. punctulatus group. Optimal and suboptimal secondary structures were investigated and revealed structures where the 5' region folded independently of the 3' region. Due to the large level of sequence variation between species, the ITS2 region proved unsuitable for phylogenetic analysis.
对尖音库蚊组中的十个隐种的核糖体DNA内转录间隔区2（ITS2）进行了测序和特征分析，该组成员是西南太平洋地区疟疾和丝虫病的主要传播媒介。ITS2的长度在549 bp至565 bp之间，由于主要是简单序列的插入缺失，其序列变异水平在2.3%至24.3%之间。GC含量在61.3%至70.9%之间变化。这些值高于在其他蚊子隐种中发现的值，仅与大劣按蚊复合体的成员相当，这表明这组亚洲蚊子与尖音库蚊组之间可能存在联系。研究了最佳和次佳二级结构，发现其结构中5'区域与3'区域独立折叠。由于物种间序列变异水平较高，ITS2区域被证明不适用于系统发育分析。
