Sakaguchi M, Hashimoto M, Nigi H, Yasueda H, Takahashi Y, Watanabe M, Nagoya T, Taniguchi Y, Kurimoto M, Inouye S
Department of Epidemiology, National Institute of Health, Tokyo, Japan.
Immunology. 1997 Jun;91(2):161-6. doi: 10.1046/j.1365-2567.1997.00255.x.
Japanese cedar (Cryptomeria japonica) pollinosis has been reported to occur naturally in Japanese monkeys (Macaca fuscata) as well as humans. Using monoclonal antibodies (mAb) specific to Cry j 1, a major allergen in Japanese cedar pollen, we identified five independent epitopes (EP-1 to EP-5) on the molecule. The epitopes recognized by IgE antibodies in the sera of humans and monkeys with the pollinosis were analysed by an IgE enzyme-linked immunosorbent assay inhibition method with these mAb. In human patients, the mAb to EP-1 strongly blocked the binding of IgE antibodies in all patients' sera to Cry j 1. The reaction patterns of IgE antibodies in monkeys, however, varied among the troops of monkeys. In some troops, the mAb to EP-1 showed a blocking pattern similar to that for human patients. In other troops, mAb to EP-4 and EP-5 blocked binding of IgE. These results indicate that some, but not all, monkeys have antibody responses to the major allergen similar to those of humans.
据报道，日本猕猴（Macaca fuscata）以及人类都会自然发生日本柳杉（Cryptomeria japonica）花粉症。利用针对日本柳杉花粉中的主要过敏原Cry j 1的单克隆抗体（mAb），我们在该分子上鉴定出了五个独立的表位（EP-1至EP-5）。采用这些单克隆抗体，通过IgE酶联免疫吸附测定抑制法分析了患有花粉症的人类和猕猴血清中IgE抗体识别的表位。在人类患者中，针对EP-1的单克隆抗体强烈阻断了所有患者血清中IgE抗体与Cry j 1的结合。然而，猕猴中IgE抗体的反应模式在不同猴群中有所不同。在一些猴群中，针对EP-1的单克隆抗体表现出与人类患者相似的阻断模式。在其他猴群中，针对EP-4和EP-5的单克隆抗体阻断了IgE的结合。这些结果表明，部分而非全部猕猴对主要过敏原的抗体反应与人类相似。
