Bowman P D, Schuschereba S T, Lawlor D F, Gilligan G R, Mata J R, DeBaere D R
United States Army Institute of Surgical Research, Fort Sam, Houston 78234-6315, USA.
Am J Physiol. 1997 Jun;272(6 Pt 1):C1988-94. doi: 10.1152/ajpcell.1997.272.6.C1988.
Thermal injury by short pulses (1-30 s) of relatively high temperature (50-68 degrees C) was investigated in normal human epidermal keratinocytes (NHEK). NHEK were cultured on plastic cover-slips and dipped in medium held at various temperatures. Survival assessed by methylthiazol tetrazolium reduction assay at 6 days postheating demonstrated an inverse time-temperature relationship that indicated that most cells could survive after a 1-s, 60 degrees C exposure or a 30-s, 55 degrees C exposure. Arrhenius plots of the data indicated major transition points for cell injury at 50 and 60 degrees C. Heat shock protein 70 (HSP70) and interleukin-8 (IL-8) were both induced by elevation of temperature between 50 and 60 degrees C for as short a time as 1 s. HSP70 synthesis stimulated by short, high pulses of heat appeared to induce thermotolerance. These results demonstrate that brief exposure to relatively high temperature can induce HSP70 and IL-8 synthesis in keratinocytes.
在正常人表皮角质形成细胞（NHEK）中研究了相对高温（50 - 68摄氏度）的短脉冲（1 - 30秒）造成的热损伤。将NHEK培养在塑料盖玻片上，并浸入保持在不同温度的培养基中。在加热后6天通过噻唑蓝还原试验评估细胞存活情况，结果显示出时间 - 温度的反比关系，这表明大多数细胞在60摄氏度暴露1秒或55摄氏度暴露30秒后能够存活。数据的阿累尼乌斯图表明在50和60摄氏度时细胞损伤有主要转变点。热休克蛋白70（HSP70）和白细胞介素 - 8（IL - 8）在50至60摄氏度之间温度升高仅1秒时均被诱导。短时间、高强度热脉冲刺激的HSP70合成似乎诱导了热耐受性。这些结果表明，短暂暴露于相对高温可诱导角质形成细胞中HSP70和IL - 8的合成。
