Killian G J, Snyder J, Amann R P
Cell Tissue Res. 1977 Sep 30;183(3):371-8. doi: 10.1007/BF00220643.
Cells from the rat caput epididymidis were separated by unit gravity sedimentation. Purest 12-ml fractions contained 88-95% basal cells or 64-76% principal cells. Ultrastructure of separated cells was similar to that of cells in intact tissue. Viability of separated cells was excellent as determined by dye exclusion tests and cellular ATP content. By combining fractions pools containing 4.0 +/- 0.9 X 10(6) cells (86 +/- 8% basal cells) and 1.4 +/- 0.4 X 10(6) cells (56 +/- 7% principal cells) were obtained. Thus, studies on the function of basal and principal cells from the rat caput epididymidis should be possible.
大鼠附睾头细胞通过单位重力沉降法进行分离。最纯的12毫升级分含有88 - 95%的基底细胞或64 - 76%的主细胞。分离细胞的超微结构与完整组织中的细胞相似。通过染料排斥试验和细胞ATP含量测定，分离细胞的活力极佳。通过合并级分池，获得了含有4.0±0.9×10⁶个细胞（86±8%基底细胞）和1.4±0.4×10⁶个细胞（56±7%主细胞）的细胞群。因此，对大鼠附睾头基底细胞和主细胞功能的研究应该是可行的。
