Figueras J B, Garcia-Gil L J, Abella C A
Laboratory of Microbiology, University of Girona, Faculty of Science, Spain.
FEMS Microbiol Lett. 1997 Jul 1;152(1):31-6. doi: 10.1111/j.1574-6968.1997.tb10405.x.
A comparative analysis of 16S ribosomal DNA sequences from 18 strains belonging to different species of the genus Chlorobium has been made in order to investigate their phylogenetic relationships. All studied strains with brown pigmentation, belonging to species C. phaeobacteroides and C. phaeovibrioides, were clustered together and separated from green species. Most of the C. limicola strains formed a consistent group and were clustered next to C. tepidum. But, despite their identical morphology, C. vibrioforme strain 6030 (= DSM 260T) and C. vibrioforme strain 8327 (= DSM 263) were clearly differentiated on the basis of their 16S rDNA sequence. On the other hand, morphologically distinctive strains of C. chlorovibrioides, C. vibrioforme and C. limicola presented sequences with a high degree of similarity. Since morphological characters do not seem to be sufficient to adequately classify this group, these findings help to set up the basis for a revision of the taxonomy of the genus Chlorobium.
为了研究绿菌属不同物种的18个菌株之间的系统发育关系，对其16S核糖体DNA序列进行了比较分析。所有研究的具有棕色色素沉着的菌株，属于噬纤维绿菌和噬颤绿菌物种，聚集在一起并与绿色物种分开。大多数嗜盐绿菌菌株形成了一个一致的群体，并紧挨着嗜热栖热绿菌聚集。但是，尽管嗜颤绿菌菌株6030（=DSM 260T）和嗜颤绿菌菌株8327（=DSM 263）形态相同，但根据它们的16S rDNA序列却明显区分开来。另一方面，形态上有明显差异的绿颤绿菌、嗜颤绿菌和嗜盐绿菌菌株呈现出高度相似的序列。由于形态特征似乎不足以对该类群进行充分分类，这些发现有助于为绿菌属分类学的修订奠定基础。
