do Valle G R, Gomes T A, Irino K, Trabulsi L R
Seção de Bacteriologia, Instituto Adolfo Lutz, São Paulo, Brazil.
FEMS Microbiol Lett. 1997 Jul 1;152(1):95-100. doi: 10.1111/j.1574-6968.1997.tb10414.x.
Genotypic and phenotypic virulence markers of the different categories of diarrheagenic Escherichia coli were investigated in 76 strains of the enteropathogenic E. coli (EPEC) serogroup O125. The most frequent serotype found was O125ac:H21. None of the serotypes behaved as EPEC, i.e. carried the eaeA, bfpA, and EAF DNA sequences simultaneously and presented localized adherence to HeLa cells. All strains of O125ac:H6 were atypical EPEC since they carried eaeA only, and presented an indefinite pattern of adherence. All strains of O125ab:H9, O125ac:H9, O125?:H16, and O125ab:H21 and 79% of the O125ac:H21 strains were enteroaggregative E. coli, since they carried a specific DNA sequence and presented the typical aggregative adherence pattern.
对76株肠致病性大肠杆菌(EPEC)血清群O125的不同类别致泻性大肠杆菌的基因型和表型毒力标记进行了研究。最常见的血清型是O125ac:H21。没有一种血清型表现为EPEC,即不同时携带eaeA、bfpA和EAF DNA序列,也未呈现对HeLa细胞的局部黏附。所有O125ac:H6菌株均为非典型EPEC,因为它们仅携带eaeA,且呈现不确定的黏附模式。所有O125ab:H9、O125ac:H9、O125?:H16和O125ab:H21菌株以及79%的O125ac:H21菌株都是肠集聚性大肠杆菌,因为它们携带特定的DNA序列并呈现典型的集聚性黏附模式。