Konate G, Traore O, Coulibaly M M
Institut d'Etudes et de Recherches Agricoles Ougadougou, Burkina Faso.
Arch Virol. 1997;142(6):1117-24. doi: 10.1007/s007050050146.
The use of a panel of polyclonal antisera and monoclonal antibodies (MAbs) raised against West African isolates of rice yellow mottle virus (RYMV) in ELISA resulted in separation of 73 RYMV isolates into three distinct serogroups. Using a set of differential rice varieties, the serogroups could be correlated to two RYMV pathotypes. A relationship was found between serological properties of the RYMV isolates and their probable ecological origin. It was concluded that RYMV isolates originating in closely related agroecological zones displayed variability in coat protein and pathogenicity. This should be taken into account in developing tolerant or resistant rice varieties.
利用一组针对西非水稻黄斑驳病毒(RYMV)分离株制备的多克隆抗血清和单克隆抗体(MAb)进行酶联免疫吸附测定(ELISA),可将73个RYMV分离株分为三个不同的血清群。使用一组鉴别水稻品种,这些血清群可与两种RYMV致病型相关联。发现RYMV分离株的血清学特性与其可能的生态起源之间存在关联。得出的结论是,源自密切相关农业生态区的RYMV分离株在外壳蛋白和致病性方面表现出变异性。在培育耐病或抗病水稻品种时应考虑到这一点。