Bockhard H, Oelschläger H, Pooth R
Institut für Pharmazie, Friedrich-Schiller-Universität, Jena, Germany.
Pharmazie. 1997 May;52(5):357-61.
After oral doses of piracetam this drug is detectable in serum as well as in urine after formation of the red Fe(III)-hydroxamatecomplex by thin-layer densitometry. Detection in serum requires extraction with a mixture of dichloromethane/ methanol, while urine can be used directly after dilution. The limit of quantification in urine is 100 micrograms/ml and in serum 4.0 micrograms/ml. The new method was validated by HPLC. The coefficient of correlation was 0.9999% for determination in urine and 0.9986% for determination in serum.
口服吡拉西坦后，通过薄层光密度法形成红色Fe(III)-异羟肟酸络合物后，该药物在血清和尿液中均可检测到。血清检测需要用二氯甲烷/甲醇混合物萃取，而尿液稀释后可直接使用。尿液中的定量限为100微克/毫升，血清中的定量限为4.0微克/毫升。该新方法通过高效液相色谱法进行了验证。尿液测定的相关系数为0.9999%，血清测定的相关系数为0.9986%。
