Hänsler M, Jakubke H D
Faculty of Biosciences, Pharmacy and Psychology, Leipzig University, Germany.
J Pept Sci. 1996 Sep-Oct;2(5):279-89. doi: 10.1002/psc.65.
During the past decade proteases have been widely used as catalysts in peptide synthesis. Unfortunately, they are not ideal ligases. Enzymatic peptide synthesis in frozen aqueous systems has been developed as an approach towards the suppression of competitive reactions. This paper summarizes reports concerning the behaviour of non-enzymatic as well as of enzyme-catalysed reactions when the reaction mixture is frozen. The advantages of freezing the reaction mixture in serine and cysteine protease-catalysed peptide synthesis, the influence of modified reaction conditions and the possible reasons for the yield-increasing effect of freezing are discussed.
在过去十年中，蛋白酶已被广泛用作肽合成的催化剂。不幸的是，它们并非理想的连接酶。冷冻水体系中的酶促肽合成已被开发为一种抑制竞争反应的方法。本文总结了有关反应混合物冷冻时非酶促反应以及酶催化反应行为的报告。讨论了在丝氨酸和半胱氨酸蛋白酶催化的肽合成中冷冻反应混合物的优点、改性反应条件的影响以及冷冻产率增加效应的可能原因。
