Wu L, Siddiqui A, Morris D E, Cox D A, Roth S I, Mustoe T A
Division of Plastic and Reconstructive Surgery, Northwestern University Medical School, Chicago, Ill, USA.
Arch Surg. 1997 Jul;132(7):753-60. doi: 10.1001/archsurg.1997.01430310067014.
Transforming growth factor (TGF) beta 3 is a new isoform of the TGF beta superfamily and is presumed to play an important role in wound repair and scarring.
To examine the effects of TGF beta 3 on wound healing and on reducing scarring.
Dermal ulcers were created on the ears of 75 anesthetized young female rabbits. Either TGF beta 3 or vehicle was applied topically to the wounds. Wounds were bisected and analyzed histologically at postwounding day 7. A second group of wounds was treated with topical TGF beta 3 and TGF beta 2 or vehicle at days 0 and 3 and harvested at days 21 through 42 as an excessive scarring model. The third group of wounds was treated with TGF beta 1, TGF beta 2, and TGF beta 3 and vehicle. The granulation tissue was harvested at day 7, and cellular RNA was extracted for performing competitive reverse-transcription polymerase chain reaction.
The amount of new epithelium and granulation tissue was measured in TGF beta 3- and vehicle-treated wounds. The hypertrophic index was calculated for scarring wounds treated with TGF beta 2 and TGF beta 3 or vehicle. Levels of TGF beta 1 messenger RNA were measured in those wounds that were treated with TGF beta 1, TGF beta 2, and TGF beta 3 and in their controls.
The use of TGF beta 3 (0.3-0.75 microgram per wound) increased granulation tissue formation by more than 100% (P < .005). Epithelialization showed a biphase, either increasing 30% (P < .04) or decreasing 25% (P < .001) dependent on dose. No significant difference in the hypertrophic index was noted in TGF beta 3-treated wounds compared with controls. Levels of TGF beta 1 messenger RNA increased (7.1- to 14.9-fold) in those wounds treated with TGF beta s compared with controls at day 7.
Exogenous TGF beta 3 displays substantial vulnerary properties in wound healing and may be useful in treating nonhealing wounds. However, the observation that TGF beta 3 can reduce scarring was not confirmed in this study, and the messenger RNA level in response to TGF beta 3 suggests that it behaves similarly to TGF beta 1.
转化生长因子（TGF）β3是TGF-β超家族的一种新亚型，推测在伤口修复和瘢痕形成中起重要作用。
研究TGF-β3对伤口愈合及减少瘢痕形成的作用。
在75只麻醉的年轻雌性兔耳朵上制造皮肤溃疡。将TGF-β3或赋形剂局部应用于伤口。在伤后第7天，将伤口一分为二并进行组织学分析。第二组伤口在第0天和第3天用局部TGF-β3和TGF-β2或赋形剂处理，并在第21天至42天作为过度瘢痕形成模型进行取材。第三组伤口用TGF-β1、TGF-β2和TGF-β3及赋形剂处理。在第7天采集肉芽组织，提取细胞RNA用于竞争性逆转录聚合酶链反应。
测量TGF-β3处理组和赋形剂处理组伤口中新上皮和肉芽组织的量。计算用TGF-β2和TGF-β3或赋形剂处理的瘢痕形成伤口的肥厚指数。测量用TGF-β1、TGF-β2和TGF-β3处理的伤口及其对照组中TGF-β1信使核糖核酸的水平。
使用TGF-β3（每伤口0.3 - 0.75微克）可使肉芽组织形成增加超过100%（P <.005）。上皮化呈双相性，根据剂量不同，要么增加30%（P <.04），要么减少25%（P <.001）。与对照组相比，TGF-β3处理的伤口在肥厚指数上无显著差异。在第7天，与对照组相比，用TGF-βs处理的伤口中TGF-β1信使核糖核酸水平增加（7.1至14.9倍）。
外源性TGF-β3在伤口愈合中显示出显著的促进愈合特性，可能对治疗不愈合伤口有用。然而，本研究未证实TGF-β3能减少瘢痕形成这一观察结果，且对TGF-β3反应的信使核糖核酸水平表明其行为与TGF-β1相似。
