Oosumi T, Belknap W R
United States Department of Agriculture, Agricultural Research Service, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USA.
J Mol Evol. 1997 Aug;45(2):137-44. doi: 10.1007/pl00006213.
Sol3 transposons are mobile elements defined by long terminal inverted repeats which are found in tomato and potato. Members of the Sol3 family have been isolated from a variety of solanaceous species including Solanum tuberosum (potato), S. demissum, S. chacoense, Lycopersicon esculentum (tomato), and L. hirsutum. While highly conserved elements are found within different species, Sol3 terminal inverted repeats can also flank unrelated sequences. Southern blot analysis indicates that Sol3 elements are less prevalent in the potato (approximately 50 copies) than in the tomato (>100 copies) genome. No Sol3-hybridizing sequences were observed in tobacco. While a number of Sol3 elements ranging in size from 500 bp to 2 kbp were sequenced, no transposase coding domains could be identified within the internal regions of the elements. The data suggest that the Sol3 represent a heterogeneous family of nonautonomous transposable elements associated with an as-yet-unidentified autonomous transposon.
Sol3转座子是由长末端反向重复序列定义的可移动元件，存在于番茄和马铃薯中。Sol3家族的成员已从多种茄科物种中分离出来，包括马铃薯（Solanum tuberosum）、德米氏茄（S. demissum）、查科茄（S. chacoense）、番茄（Lycopersicon esculentum）和多毛番茄（L. hirsutum）。虽然在不同物种中发现了高度保守的元件，但Sol3末端反向重复序列也可以位于不相关的序列两侧。Southern杂交分析表明，Sol3元件在马铃薯基因组中（约50个拷贝）比在番茄基因组中（>100个拷贝）更不常见。在烟草中未观察到与Sol3杂交的序列。虽然对一些大小从500 bp到2 kbp的Sol3元件进行了测序，但在元件的内部区域未发现转座酶编码结构域。数据表明，Sol3代表了一个与尚未鉴定的自主转座子相关的非自主转座元件的异质家族。
