Department of Molecular Biology, University of California, Berkeley, CA 94720, USA.
EMBO J. 1986 Dec 20;5(13):3615-23. doi: 10.1002/j.1460-2075.1986.tb04690.x.
The hobo transposable elements of Drosophila form a family of 3.0-kb elements and their deletion derivatives. Their distribution is consistent with the model that 3.0-kb elements are functionally complete but that smaller hobos are defective and require complete elements in trans for transposition. The sequence of one 3.0-kb element is presented; it has several interesting features, including a 1.9-kb open reading frame downstream from potential TATA and CAT sequences. Comparison of 11 independent insertion sites shows that in every case the hobo element has integrated at and duplicated either the sequence NNNNNNAC or CTTTNNNN. There is evidence that an eight nucleotide sequence internal to hobo that matches both of these sequences has been used as an insertion site for a second hobo element, as the first step in the creation of an internal deletion derivative. Structural similarities between hobo and the eukaryotic transposable elements P, Ac, 1723, and Tam3, found in widely divergent host organisms, suggest that they all transpose by a common mechanism.
果蝇中的流浪汉转座子形成了一个 3.0kb 元件及其缺失衍生物家族。它们的分布与这样的模型一致，即 3.0kb 元件是功能完整的，但较小的流浪汉是有缺陷的，需要完整的元件在转座中才能转座。本文呈现了一个 3.0kb 元件的序列；它具有几个有趣的特征，包括在潜在的 TATA 和 CAT 序列下游有一个 1.9kb 的开放阅读框。对 11 个独立的插入位点的比较表明，在每种情况下，霍伯元件都整合在 NNNNNNAC 或 CTTTNNNN 序列上并进行了复制。有证据表明，霍伯内部的一个 8 个核苷酸序列与这两个序列都匹配，已被用作第二个霍伯元件的插入位点，作为创建内部缺失衍生物的第一步。在广泛不同的宿主生物中发现的霍伯与真核转座元件 P、Ac、1723 和 Tam3 之间的结构相似性表明，它们都通过共同的机制进行转座。
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