Dessaint J P, Camus D, Fischer E, Capron A
Eur J Immunol. 1977 Sep;7(9):624-9. doi: 10.1002/eji.1830070909.
Normal spleen cells of CBA mice or Fischer rats were cultured with mitogens or allogeneic cells, together with various substances of Schistosoma mansoni origin, and thymidine uptake was measured. The proliferation (DNA synthesis) of normal lymphocytes was inhibited by the incubation product of the parasite as well as by cell-free supernatant of schistosome culture. Inhibition was obtained only when active materials were added at the beginning of the culture. Both T and B cell proliferation were inhibited. The inhibitory activity found in cell-free supernatant suggested the release by the parasite of some factor(s) interfering with lymphocyte proliferation. Moreover, serum from rats infected by S. mansoni inhibited lymphocyte proliferation also. The inhibitor(s) appeared heat resistant, dialyzable and of low molecular weight (500-1000). Incubation of normal spleen cells with S. mansoni inhibitor(s) did not enhance the release of nonspecific suppressor cell factor. The inhibition of product(s) released by the parasite could explain part of the immunosuppression status found in schistosomiasis.
将CBA小鼠或Fischer大鼠的正常脾细胞与丝裂原或同种异体细胞一起，以及与曼氏血吸虫来源的各种物质共同培养，并测定胸腺嘧啶核苷摄取量。寄生虫的培养产物以及血吸虫培养的无细胞上清液均可抑制正常淋巴细胞的增殖（DNA合成）。只有在培养开始时添加活性物质才能获得抑制作用。T细胞和B细胞的增殖均受到抑制。在无细胞上清液中发现的抑制活性表明寄生虫释放了一些干扰淋巴细胞增殖的因子。此外，感染曼氏血吸虫的大鼠血清也能抑制淋巴细胞增殖。该抑制剂似乎具有耐热性、可透析性且分子量较低（500-1000）。用曼氏血吸虫抑制剂培养正常脾细胞不会增强非特异性抑制细胞因子的释放。寄生虫释放产物的抑制作用可以解释血吸虫病中发现的部分免疫抑制状态。
