Castellanos J E, Castañeda D R, Velandia A E, Hurtado H
Laboratorio de Neurociencias, Instituto Nacional de Salud, Santafé de Bogotá, Columbia.
Neurosci Lett. 1997 Jul 4;229(3):198-200. doi: 10.1016/s0304-3940(97)00440-0.
The infection of target cells by rabies virus is effected through membrane receptors. Several authors have suggested that nicotinic receptors could be used by this virus, but no direct experimental evidence is available. In this study mouse dorsal root ganglia cells were treated with various nicotinic antagonists (dihydro-beta-eritroidine, mecamilamine, d-tubocurarin, hexametonium, alpha-bungarotoxin and erabutoxin). After incubation, the cultures were infected with rabies virus. Cells were fixed, and processed for immunodetection of rabies virus. Treatment with mecamilamine or d-tubocurarine reduced the percentage of infected neurons. None of the antagonists tested changed the percentage of infected non-neuronal cells.
狂犬病病毒对靶细胞的感染是通过膜受体实现的。几位作者提出，该病毒可能利用烟碱型受体，但尚无直接的实验证据。在本研究中，用各种烟碱型拮抗剂（二氢-β-刺桐啶、美加明、d-筒箭毒碱、六甲铵、α-银环蛇毒素和海蛇毒素）处理小鼠背根神经节细胞。孵育后，用狂犬病病毒感染培养物。细胞固定后，进行狂犬病病毒的免疫检测。用美加明或d-筒箭毒碱处理可降低被感染神经元的百分比。所测试的拮抗剂均未改变被感染非神经元细胞的百分比。
