与粟酒裂殖酵母着丝粒K型重复DNA结合的CENP - B同源蛋白的纯化与鉴定
Purification and characterization of a CENP-B homologue protein that binds to the centromeric K-type repeat DNA of Schizosaccharomyces pombe.
作者信息
Lee J K, Huberman J A, Hurwitz J
机构信息
Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
出版信息
Proc Natl Acad Sci U S A. 1997 Aug 5;94(16):8427-32. doi: 10.1073/pnas.94.16.8427.
Abstract
We have purified and characterized a novel 60-kDa protein that binds to centromeric K-type repeat DNA from Schizosaccharomyces pombe. This protein was initially purified by its ability to bind to the autonomously replicating sequence 3002 DNA. Cloning of the gene encoding this protein revealed that it possesses significant homology to the mammalian centromere DNA-binding protein CENP-B and S. pombe Abp1, and this gene was designated as cbh+ (CENP-B homologue). Cbh protein specifically interacts in vitro with the K-type repeat DNA, which is essential for centromere function. The Cbh-binding consensus sequence was determined by DNase I footprinting assays as PyPuATATPyPuTA, featuring an inverted repeat of the first four nucleotides. Based on its binding activity to centromeric DNA and homology to centromere proteins, we suggest that this protein may be a functional homologue of the mammalian CENP-B in S. pombe.
摘要
我们已经纯化并鉴定了一种新的60 kDa蛋白，它能与粟酒裂殖酵母的着丝粒K型重复DNA结合。该蛋白最初是通过其与自主复制序列3002 DNA的结合能力而被纯化的。编码该蛋白的基因克隆显示，它与哺乳动物着丝粒DNA结合蛋白CENP - B和粟酒裂殖酵母Abp1具有显著同源性，该基因被命名为cbh +（CENP - B同源物）。Cbh蛋白在体外与K型重复DNA特异性相互作用，而K型重复DNA对着丝粒功能至关重要。通过DNase I足迹分析确定Cbh结合共有序列为PyPuATATPyPuTA，其前四个核苷酸呈反向重复。基于其对着丝粒DNA的结合活性以及与着丝粒蛋白的同源性，我们认为该蛋白可能是粟酒裂殖酵母中哺乳动物CENP - B的功能同源物。
相似文献
1
Purification and characterization of a CENP-B homologue protein that binds to the centromeric K-type repeat DNA of Schizosaccharomyces pombe.与粟酒裂殖酵母着丝粒K型重复DNA结合的CENP - B同源蛋白的纯化与鉴定
Proc Natl Acad Sci U S A. 1997 Aug 5;94(16):8427-32. doi: 10.1073/pnas.94.16.8427.
2
Identification, purification, and molecular cloning of autonomously replicating sequence-binding protein 1 from fission yeast Schizosaccharomyces pombe.来自裂殖酵母粟酒裂殖酵母的自主复制序列结合蛋白1的鉴定、纯化及分子克隆。
Proc Natl Acad Sci U S A. 1996 Jan 9;93(1):502-7. doi: 10.1073/pnas.93.1.502.
3
A centromere DNA-binding protein from fission yeast affects chromosome segregation and has homology to human CENP-B.来自裂殖酵母的一种着丝粒DNA结合蛋白影响染色体分离，并且与人类着丝粒蛋白B具有同源性。
J Cell Biol. 1997 Feb 10;136(3):487-500. doi: 10.1083/jcb.136.3.487.
4
The centromeric K-type repeat and the central core are together sufficient to establish a functional Schizosaccharomyces pombe centromere.着丝粒K型重复序列和中央核心区域共同足以建立一个功能性的粟酒裂殖酵母着丝粒。
Mol Biol Cell. 1994 Jul;5(7):747-61. doi: 10.1091/mbc.5.7.747.
5
Fission yeast CENP-B homologs nucleate centromeric heterochromatin by promoting heterochromatin-specific histone tail modifications.裂殖酵母着丝粒蛋白B同源物通过促进异染色质特异性组蛋白尾部修饰来形成着丝粒异染色质。
Genes Dev. 2002 Jul 15;16(14):1766-78. doi: 10.1101/gad.997702.
6
Functional redundancies, distinct localizations and interactions among three fission yeast homologs of centromere protein-B.着丝粒蛋白B的三个裂殖酵母同源物之间的功能冗余、不同定位及相互作用。
Genetics. 2001 Mar;157(3):1191-203. doi: 10.1093/genetics/157.3.1191.
7
The centromere enhancer mediates centromere activation in Schizosaccharomyces pombe.着丝粒增强子介导粟酒裂殖酵母中的着丝粒激活。
Mol Cell Biol. 1997 Jun;17(6):3305-14. doi: 10.1128/MCB.17.6.3305.
8
Fission yeast homologs of human CENP-B have redundant functions affecting cell growth and chromosome segregation.人类着丝粒蛋白B（CENP-B）在裂殖酵母中的同源物具有影响细胞生长和染色体分离的冗余功能。
Mol Cell Biol. 2000 Apr;20(8):2852-64. doi: 10.1128/MCB.20.8.2852-2864.2000.
9
A NASP (N1/N2)-related protein, Sim3, binds CENP-A and is required for its deposition at fission yeast centromeres.一种与核仁酸性蛋白（NASP，N1/N2）相关的蛋白质Sim3，可结合着丝粒蛋白A（CENP-A），并且是其在裂殖酵母着丝粒上沉积所必需的。
Mol Cell. 2007 Dec 28;28(6):1029-44. doi: 10.1016/j.molcel.2007.10.010.
10
Functional cloning of centromere protein B (CENP-B) box-enriched alphoid DNA repeats utilizing the sequence-specific DNA binding activity of human CENP-B in vitro.利用人着丝粒蛋白B（CENP - B）体外序列特异性DNA结合活性对富含着丝粒蛋白B（CENP - B）盒的α卫星DNA重复序列进行功能克隆。
Chromosome Res. 1994 Nov;2(6):453-9. doi: 10.1007/BF01552868.
引用本文的文献
1
The dark side of centromeres: types, causes and consequences of structural abnormalities implicating centromeric DNA.着丝粒的阴暗面：涉及着丝粒 DNA 的结构异常的类型、原因和后果。
Nat Commun. 2018 Oct 18;9(1):4340. doi: 10.1038/s41467-018-06545-y.
2
Centromere Stability: The Replication Connection.着丝粒稳定性：复制关联
Genes (Basel). 2017 Jan 18;8(1):37. doi: 10.3390/genes8010037.
3
The fission yeast CENP-B protein Abp1 prevents pervasive transcription of repetitive DNA elements.裂殖酵母着丝粒蛋白B（CENP-B）蛋白Abp1可防止重复DNA元件的广泛转录。
Biochim Biophys Acta. 2016 Oct;1859(10):1314-21. doi: 10.1016/j.bbagrm.2016.06.009. Epub 2016 Jun 23.
4
Centromeres of filamentous fungi.丝状真菌的着丝粒。
Chromosome Res. 2012 Jul;20(5):635-56. doi: 10.1007/s10577-012-9290-3.
5
CENP-B preserves genome integrity at replication forks paused by retrotransposon LTR.CENP-B 可维持因逆转录转座子 LTR 而暂停的复制叉处的基因组完整性。
Nature. 2011 Jan 6;469(7328):112-5. doi: 10.1038/nature09608. Epub 2010 Dec 12.
6
The fission yeast homologue of CENP-B, Abp1, regulates directionality of mating-type switching.CENP-B的裂殖酵母同源物Abp1调节交配型转换的方向性。
EMBO J. 2008 Apr 9;27(7):1029-38. doi: 10.1038/emboj.2008.53. Epub 2008 Mar 20.
7
Convergent domestication of pogo-like transposases into centromere-binding proteins in fission yeast and mammals.裂殖酵母和哺乳动物中类似Pogo转座酶向着丝粒结合蛋白的趋同驯化。
Mol Biol Evol. 2008 Jan;25(1):29-41. doi: 10.1093/molbev/msm221. Epub 2007 Oct 16.
8
The CENP-B homolog, Abp1, interacts with the initiation protein Cdc23 (MCM10) and is required for efficient DNA replication in fission yeast.着丝粒蛋白B同源物Abp1与起始蛋白Cdc23（MCM10）相互作用，是裂殖酵母中高效DNA复制所必需的。
Cell Div. 2006 Nov 17;1:27. doi: 10.1186/1747-1028-1-27.
9
Chromosome segregation in fission yeast with mutations in the tubulin folding cofactor D.微管蛋白折叠辅助因子D发生突变的裂殖酵母中的染色体分离
Curr Genet. 2006 Nov;50(5):281-94. doi: 10.1007/s00294-006-0095-9. Epub 2006 Sep 27.
10
Kinetochore and heterochromatin domains of the fission yeast centromere.裂殖酵母着丝粒的动粒和异染色质结构域。
Chromosome Res. 2004;12(6):521-34. doi: 10.1023/B:CHRO.0000036586.81775.8b.
本文引用的文献
1
A centromere DNA-binding protein from fission yeast affects chromosome segregation and has homology to human CENP-B.来自裂殖酵母的一种着丝粒DNA结合蛋白影响染色体分离，并且与人类着丝粒蛋白B具有同源性。
J Cell Biol. 1997 Feb 10;136(3):487-500. doi: 10.1083/jcb.136.3.487.
2
Large, complex modular structure of a fission yeast DNA replication origin.裂殖酵母DNA复制起点的大型复杂模块化结构。
Curr Biol. 1996 Apr 1;6(4):467-73. doi: 10.1016/s0960-9822(02)00514-6.
3
The Saccharomyces cerevisiae kinetochore.酿酒酵母动粒
FEBS Lett. 1996 Jun 24;389(1):70-4. doi: 10.1016/0014-5793(96)00563-7.
4
Genetic analysis of an ARS element from the fission yeast Schizosaccharomyces pombe.来自裂殖酵母粟酒裂殖酵母的一个自主复制序列元件的遗传分析。
EMBO J. 1995 Dec 15;14(24):6348-57. doi: 10.1002/j.1460-2075.1995.tb00326.x.
5
Identification, purification, and molecular cloning of autonomously replicating sequence-binding protein 1 from fission yeast Schizosaccharomyces pombe.来自裂殖酵母粟酒裂殖酵母的自主复制序列结合蛋白1的鉴定、纯化及分子克隆。
Proc Natl Acad Sci U S A. 1996 Jan 9;93(1):502-7. doi: 10.1073/pnas.93.1.502.
6
Centromeres of the fission yeast Schizosaccharomyces pombe are highly variable genetic loci.裂殖酵母粟酒裂殖酵母的着丝粒是高度可变的基因座。
Mol Cell Biol. 1993 Aug;13(8):4578-87. doi: 10.1128/mcb.13.8.4578-4587.1993.
7
Characterisation of PDC2, a gene necessary for high level expression of pyruvate decarboxylase structural genes in Saccharomyces cerevisiae.PDC2的特性研究，PDC2是酿酒酵母中丙酮酸脱羧酶结构基因高水平表达所必需的一个基因。
Mol Gen Genet. 1993 Dec;241(5-6):657-66. doi: 10.1007/BF00279908.
8
CENP-C is required for maintaining proper kinetochore size and for a timely transition to anaphase.着丝粒蛋白C对于维持着丝粒的正常大小以及及时过渡到后期是必需的。
J Cell Biol. 1994 May;125(3):531-45. doi: 10.1083/jcb.125.3.531.
9
Specific initiation at an origin of replication from Schizosaccharomyces pombe.
Mol Cell Biol. 1994 Mar;14(3):1796-805. doi: 10.1128/mcb.14.3.1796-1805.1994.
10
Role of nonhistone proteins in the chromosomal events of mitosis.非组蛋白在有丝分裂染色体事件中的作用。
FASEB J. 1994 Sep;8(12):947-56. doi: 10.1096/fasebj.8.12.8088460.
Zotero 插件