Andrews E, Fernández H, Folch H, Eller G, Pollette M
Departamento de Microbiología, Universidad de Concepción, Valdivia, Chile.
Medicina (B Aires). 1996;56(5 Pt 1):487-92.
Using a flagellated (052) and an aflagellated (T-1) strains we studied the participation of flagellar epitopes of C. jejuni in the adhesion to HEp-2 cells in vitro. Strain 052 was significantly more adherent than strain T-1. When adhesion assays were carried out with antiflagella monoclonal antibodies, strain 052 showed inhibition of their adhesive capacity that varied between 64.3 and 92.9%. With an ELISA test it was demonstrated that those monoclonal antibodies were specific and directed exclusively against flagellar epitopes of strain 052 being unreactive with strain T-1. Our results show that flagellar epitopes could participate in the adhesion process suggesting that flagella could be involved in the installation of the infectious process.
我们使用一株有鞭毛的（052）和一株无鞭毛的（T-1）空肠弯曲菌菌株，研究了空肠弯曲菌鞭毛表位在体外对HEp-2细胞黏附中的作用。052菌株的黏附能力显著高于T-1菌株。当使用抗鞭毛单克隆抗体进行黏附试验时，052菌株的黏附能力受到抑制，抑制率在64.3%至92.9%之间。通过酶联免疫吸附测定（ELISA）试验证明，这些单克隆抗体具有特异性，仅针对052菌株的鞭毛表位，与T-1菌株无反应。我们的结果表明，鞭毛表位可能参与黏附过程，提示鞭毛可能参与感染过程的起始。
