Fernández H, Folch H, Eller G, Navarrete N, Andrews E, Gajardo T
Institutos de Microbiología Clínica, Inmunología y Parasitología, Universidad Austral de Chile, Valdivia, Chile.
Rev Med Chil. 1996 Sep;124(9):1029-35.
The participation of the flagella of a virulent strain (O52) of Campylobacter jejuni subsp. jejuni in the adhesion to HEp-2 cells and their inhibition by means of homologous polyclonal antibodies, monoclonal anti-flagella antibodies and colostral natural antibodies (IgA) was studied. An aflagellated strain (T1) was used as negative control. Adhesion was observed in higher rates with O52 strain (72%) than with T1 strain (27.5%). Polyclonal, monoclonal and colostral antibodies inhibited O52 strain adhesion in more than 70% (p < 0.001). T1 strain adhesion was inhibited only by polyclonal and colostral natural antibodies. Our results suggest that the flagella of C. jejuni subsp. jejuni could participate effectively in the adhesion process. However, the inhibition of T1 strain by polyclonal and colostral antibodies suggests the existence of other binds of adhesins in the bacterial surface.
研究了空肠弯曲菌空肠亚种强毒株（O52）的鞭毛在黏附到人喉表皮癌细胞（HEp-2）中的作用，以及同源多克隆抗体、单克隆抗鞭毛抗体和初乳天然抗体（IgA）对其黏附的抑制作用。以无鞭毛菌株（T1）作为阴性对照。观察到O52菌株的黏附率（72%）高于T1菌株（27.5%）。多克隆抗体、单克隆抗体和初乳抗体对O52菌株黏附的抑制率超过70%（p < 0.001）。T1菌株的黏附仅被多克隆抗体和初乳天然抗体抑制。我们的结果表明，空肠弯曲菌空肠亚种的鞭毛可有效参与黏附过程。然而，多克隆抗体和初乳抗体对T1菌株的抑制表明细菌表面存在其他黏附素结合位点。
