Farhi J, Nahum H, Zakut H, Levran D
Wolfson Medical Center, Holon, Israel.
Fertil Steril. 1997 Aug;68(2):318-22. doi: 10.1016/s0015-0282(97)81522-1.
To evaluate the effect of sperm in the culture medium on the rate of oocyte maturation in vitro from the germinal vesicle to the M2 stage.
Prospective randomized controlled study.
The IVF Unit, Wolfson Medical Center, Holon, Israel.
PATIENT(S): All women in whom oocytes were retrieved at the germinal vesicle stage between December 1995 and March 1996.
INTERVENTION(S): Oocytes retrieved at the germinal vesicle stage were divided prospectively and randomly into four groups of incubation conditions: group 1, intact germinal vesicle with cumulus; group 2, intact germinal vesicle with sperm cells in the culture medium; group 3, stripped germinal vesicle; and group 4, stripped germinal vesicle with sperm cells. Oocytes were observed 24 hours after retrieval, and the stage of maturation was recorded. Oocytes that reached the M2 stage underwent the intracytoplasmic injection procedure, and the fertilization rate in each group was recorded at 48 hours.
MAIN OUTCOME MEASURE(S): Maturation rate from the germinal vesicle to M2 stage and fertilization rate.
RESULT(S): Each group contained 20 germinal vesicle oocytes. In groups 1 and 2, 2 (10%) and 9 (45%) oocytes, respectively, reached the M2 stage at 24 hours; at 48 hours, 1 (5%) and 8 (40%) embryos developed, respectively. The results in group 2 were significantly higher than in group 1. In groups 3 and 4, 6 (30%) and 16 (80%) oocytes, respectively, reached the M2 stage at 24 hours; at 48 hours, 5 (25%) and 14 (70%) embryos developed, respectively. Results in group 4 were significantly higher than those in groups 1, 2, and 3.
CONCLUSION(S): Both methods of oocyte activation (i.e., addition of sperm to the culture medium or removal of the cumulus) enhance oocyte maturation in vitro, but the sperm-incubation method has a more pronounced effect. A combination of both methods leads to an exceptionally high rate of oocyte maturation, followed by a high fertilization rate.
评估培养基中的精子对卵母细胞体外从生发泡期到M2期成熟率的影响。
前瞻性随机对照研究。
以色列霍隆沃尔夫森医疗中心体外受精科。
1995年12月至1996年3月间在生发泡期取卵的所有女性。
将生发泡期取出的卵母细胞前瞻性随机分为四组培养条件：第1组，带有卵丘的完整生发泡；第2组，培养基中带有精子细胞的完整生发泡；第3组，去除卵丘的生发泡；第4组，去除卵丘并带有精子细胞的生发泡。取卵后24小时观察卵母细胞，并记录成熟阶段。达到M2期的卵母细胞进行胞浆内注射程序，并在48小时记录每组的受精率。
从生发泡期到M2期的成熟率和受精率。
每组有20个生发泡期卵母细胞。在第1组和第2组中，分别有2个（10%）和9个（45%）卵母细胞在24小时时达到M2期；在48小时时，分别有1个（5%）和8个（40%）胚胎发育。第2组的结果显著高于第1组。在第3组和第4组中，分别有6个（30%）和16个（80%）卵母细胞在24小时时达到M2期；在48小时时，分别有5个（25%）和14个（70%）胚胎发育。第4组的结果显著高于第1、2和3组。
两种卵母细胞激活方法（即向培养基中添加精子或去除卵丘）均可提高卵母细胞的体外成熟率，但精子孵育法效果更显著。两种方法联合使用可导致极高的卵母细胞成熟率，随后受精率也很高。
