Freyaldenhoven B S, Freyaldenhoven M P, Iacovoni J S, Vogt P K
The Scripps Research Institute, Department of Molecular & Experimental Medicine, La Jolla, California 92037, USA.
Oncogene. 1997 Jul 24;15(4):483-8. doi: 10.1038/sj.onc.1201189.
The chicken winged helix proteins, CWH-1, CWH-2 and CWH-3, were isolated and identified by homology cloning using the winged helix sequence of the retroviral oncogene qin as a probe. The CWH proteins act as growth stimulators in chicken embryo fibroblasts and in this activity resemble the Qin protein. Qin is a transcriptional regulator that functions as a repressor, and its oncogenic potential is correlated with the ability to repress transcription. In this communication we show that CWH proteins are localized in the cell nucleus, recognize the Qin DNA binding site and also function as transcriptional repressors. The repression activity of CWH-3 was mapped to the region of amino acids 211 to 311, a domain that is homologous to the major repression domain of Qin.
通过使用逆转录病毒癌基因qin的翼状螺旋序列作为探针进行同源克隆，分离并鉴定了鸡翅状螺旋蛋白CWH-1、CWH-2和CWH-3。CWH蛋白在鸡胚成纤维细胞中作为生长刺激因子起作用，并且在这种活性方面类似于Qin蛋白。Qin是一种作为阻遏物发挥作用的转录调节因子，其致癌潜力与抑制转录的能力相关。在本通讯中，我们表明CWH蛋白定位于细胞核，识别Qin DNA结合位点，并且也作为转录阻遏物发挥作用。CWH-3的阻遏活性被定位到氨基酸211至311区域，该区域与Qin的主要阻遏结构域同源。
