Pemberton P W, Lobley R W, Holmes R, Sørensen S H, Batt R M
Department of Gastroenterology, Manchester Royal Infirmary.
Res Vet Sci. 1997 Mar-Apr;62(2):191-3. doi: 10.1016/s0034-5288(97)90146-6.
This study investigated whether gluten-sensitive enteropathy (GSE) in Irish setter dogs was associated with underlying structural abnormalities of microvillar membrane proteins. Jejunal biopsies taken from eight-month-old GSE-affected dogs reared on a normal, gluten-containing diet exhibited partial villous atrophy and contained more intra-epithelial lymphocytes than controls. The morphological abnormalities were reversed by feeding a gluten-free diet for five months and the changes were accompanied by an increase in the mucosal activity of the microvillar hydrolases, particularly aminopeptidase N and dipeptidyl aminopeptidase IV, which reverted to pre-treatment levels after a gluten challenge. Two-dimensional electrophoresis of microvillar membrane proteins isolated from GSE-affected dogs revealed an essentially normal protein map that was comparable to controls. The exception was an intense 85 kDa protein spot that diminished when the affected dogs were fed a gluten-free diet and re-intensified after a gluten challenge.
本研究调查了爱尔兰雪达犬的麸质敏感性肠病（GSE）是否与微绒毛膜蛋白的潜在结构异常有关。从以正常含麸质饮食饲养的8个月大的GSE患犬获取的空肠活检组织显示出部分绒毛萎缩，且上皮内淋巴细胞比对照组更多。通过喂食无麸质饮食5个月，形态学异常得到逆转，并且这些变化伴随着微绒毛水解酶（特别是氨肽酶N和二肽基肽酶IV）的黏膜活性增加，在麸质激发后又恢复到治疗前水平。从GSE患犬分离的微绒毛膜蛋白的二维电泳显示，其蛋白质图谱基本正常，与对照组相当。唯一的例外是一个强烈的85 kDa蛋白斑点，当患犬喂食无麸质饮食时该斑点变小，在麸质激发后又重新变强。
