Hall E J, Batt R M
Department of Veterinary Pathology, University of Liverpool.
Gut. 1991 Jul;32(7):749-53. doi: 10.1136/gut.32.7.749.
Intestinal permeability to 51Cr-EDTA was examined during the development of gluten sensitive enteropathy in dogs bred from affected Irish setters and reared on a normal wheat containing diet. Comparisons were made with litter mates reared on a gluten free diet and with a control group of age matched, clinically healthy Irish setters reared on the normal diet. Studies at 4, 6, 8, and 12 months of age were correlated with morphometric and biochemical examinations of peroral jejunal biopsy specimens. Permeability was increased at all ages in the group fed gluten free diet compared with control dogs, although there were no differences in villus height, intraepithelial lymphocyte density, and alkaline phosphatase activity. At four months, permeability in the normal diet group was greater than in controls, although comparable with that in the gluten free diet group. Permeability in the normal diet group increased further in conjunction with the development of partial villus atrophy and reduced alkaline phosphatase activity, and by 12 months permeability was significantly greater than in their gluten free diet litter mates and the control dogs. The findings suggest that an underlying permeability abnormality may be involved in the pathogenesis of gluten sensitive enteropathy in Irish setter dogs.
在患有麸质敏感肠病的爱尔兰雪达犬所繁殖的幼犬，并以含正常小麦的饮食饲养的过程中，检测了其肠道对51Cr-乙二胺四乙酸（51Cr-EDTA）的通透性。将其与以无麸质饮食饲养的同窝幼犬以及以正常饮食饲养的年龄匹配、临床健康的爱尔兰雪达犬对照组进行比较。对4、6、8和12月龄犬的研究结果与经口空肠活检标本的形态计量学和生化检查结果相关联。与对照犬相比，喂食无麸质饮食的犬在所有年龄段的通透性均增加，尽管绒毛高度、上皮内淋巴细胞密度和碱性磷酸酶活性并无差异。在4个月时，正常饮食组的通透性高于对照组，但与无麸质饮食组相当。随着部分绒毛萎缩的发展和碱性磷酸酶活性降低，正常饮食组的通透性进一步增加，到12个月时，其通透性显著高于无麸质饮食的同窝幼犬和对照犬。这些发现表明，潜在的通透性异常可能参与了爱尔兰雪达犬麸质敏感肠病的发病机制。
