Mizuno M, Kitafima T, Tomita M, Kuboki Y
Department of Biochemistry, School of Dentistry, Hokkaido University, Saporro, Japan.
Biochim Biophys Acta. 1996 Jan 10;1310(1):97-102. doi: 10.1016/0167-4889(95)00151-4.
In this study, we purified C-terminal propeptide of type I collagen (PICP) from the conditioned medium of osteoblastic MC3T3-E1 cells by chromatographic and Agarose gel extraction procedures. PICP was confirmed to be present in bone by Western blotting using a specific antibody, and was proved to be synthesized by osteoblasts with metabolic labeling. PICP promoted cell-attachment of osteoblastic MC3T3-E1 cells. We conclude that PICP is synthesized by osteoblasts and stored in bone, and that it plays a role in the maintenance of bone cells on bone matrix.
在本研究中，我们通过色谱和琼脂糖凝胶提取程序从成骨细胞MC3T3-E1细胞的条件培养基中纯化了I型胶原C端前肽（PICP）。使用特异性抗体通过蛋白质印迹法证实PICP存在于骨中，并通过代谢标记证明其由成骨细胞合成。PICP促进成骨细胞MC3T3-E1细胞的细胞黏附。我们得出结论，PICP由成骨细胞合成并储存于骨中，且其在骨基质上骨细胞的维持中发挥作用。
