功能性噬菌体PRD1受体的组装依赖于IncP质粒交配型形成复合体的11个基因。
Assembly of a functional phage PRD1 receptor depends on 11 genes of the IncP plasmid mating pair formation complex.
作者信息
Grahn A M, Haase J, Lanka E, Bamford D H
机构信息
Department of Biosciences, University of Helsinki, Finland.
出版信息
J Bacteriol. 1997 Aug;179(15):4733-40. doi: 10.1128/jb.179.15.4733-4740.1997.
Abstract
PRD1, a lipid-containing double-stranded DNA bacteriophage, uses the mating pair formation (Mpf) complex encoded by conjugative IncP plasmids as a receptor. Functions responsible for conjugative transfer of IncP plasmids are encoded by two distinct regions, Tra1 and Tra2. Ten Tra2 region gene products (TrbB to TrbL) and one from the Tra1 region (TraF) form the Mpf complex. We carried out a mutational analysis of the PRD1 receptor complex proteins by isolating spontaneous PRD1-resistant mutants. The mutations were distributed among the trb genes in the Tra2 region and accumulated predominantly in three genes, trbC, trbE, and trbL. Three of 307 phage-resistant mutants were weakly transfer proficient. Mutations causing a phage adsorption-deficient, transfer-positive phenotype were analyzed by sequencing.
摘要
PRD1是一种含脂质的双链DNA噬菌体，它将接合型IncP质粒编码的交配配对形成（Mpf）复合体用作受体。负责IncP质粒接合转移的功能由两个不同区域Tra1和Tra2编码。Tra2区域的10个基因产物（TrbB至TrbL）和Tra1区域的1个基因产物（TraF）形成Mpf复合体。我们通过分离自发的PRD1抗性突变体，对PRD1受体复合体蛋白进行了突变分析。这些突变分布在Tra2区域的trb基因中，并且主要集中在三个基因trbC、trbE和trbL中。307个噬菌体抗性突变体中有3个具有较弱的转移能力。通过测序分析了导致噬菌体吸附缺陷、转移阳性表型的突变。
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本文引用的文献
1
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J Bacteriol. 1996 Dec;178(23):6720-9. doi: 10.1128/jb.178.23.6720-6729.1996.
2
Enzymology of DNA transfer by conjugative mechanisms.通过接合机制进行DNA转移的酶学
Prog Nucleic Acid Res Mol Biol. 1996;54:197-251. doi: 10.1016/s0079-6603(08)60364-5.
3
DNA packaging orders the membrane of bacteriophage PRD1.DNA包装决定了噬菌体PRD1的膜结构。
EMBO J. 1995 Dec 15;14(24):6078-86. doi: 10.1002/j.1460-2075.1995.tb00298.x.
4
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5
Membrane location of the Ti plasmid VirB proteins involved in the biosynthesis of a pilin-like conjugative structure on Agrobacterium tumefaciens.参与根癌土壤杆菌中类菌毛接合结构生物合成的Ti质粒VirB蛋白的膜定位
FEMS Microbiol Lett. 1993 Aug 1;111(2-3):287-94. doi: 10.1111/j.1574-6968.1993.tb06400.x.
6
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7
In vitro replication of bacteriophage PRD1 DNA. Characterization of the protein-primed initiation site.噬菌体PRD1 DNA的体外复制。蛋白质引发起始位点的特性分析。
Nucleic Acids Res. 1993 Aug 11;21(16):3725-30. doi: 10.1093/nar/21.16.3725.
8
Requirements for mobilization of plasmids RSF1010 and ColE1 by the IncW plasmid R388: trwB and RP4 traG are interchangeable.IncW质粒R388对质粒RSF1010和ColE1的动员要求：trwB和RP4 traG可互换。
J Bacteriol. 1994 Jul;176(14):4455-8. doi: 10.1128/jb.176.14.4455-4458.1994.
9
Essential motifs of relaxase (TraI) and TraG proteins involved in conjugative transfer of plasmid RP4.参与质粒RP4接合转移的松弛酶（TraI）和TraG蛋白的必需基序。
J Bacteriol. 1994 Jul;176(14):4285-95. doi: 10.1128/jb.176.14.4285-4295.1994.
10
Complete nucleotide sequence of Birmingham IncP alpha plasmids. Compilation and comparative analysis.伯明翰IncPα质粒的完整核苷酸序列。汇编与比较分析。
J Mol Biol. 1994 Jun 24;239(5):623-63. doi: 10.1006/jmbi.1994.1404.
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