IncP质粒编码的与细胞包膜相关的DNA转移复合物可增加细胞通透性。
The IncP plasmid-encoded cell envelope-associated DNA transfer complex increases cell permeability.
作者信息
Daugelavicius R, Bamford J K, Grahn A M, Lanka E, Bamford D H
机构信息
Department of Biosciences, Biocenter, University of Helsinki, Finland.
出版信息
J Bacteriol. 1997 Aug;179(16):5195-202. doi: 10.1128/jb.179.16.5195-5202.1997.
Abstract
IncP-type plasmids are broad-host-range conjugative plasmids. DNA translocation requires DNA transfer-replication functions and additional factors required for mating pair formation (Mpf). The Mpf system is located in the cell membranes and is responsible for DNA transport from the donor to the recipient. The Mpf complex acts as a receptor for IncP-specific phages such as PRD1. In this investigation, we quantify the Mpf complexes on the cell surface by a phage receptor saturation technique. Electrochemical measurements are used to show that the Mpf complex increases cell envelope permeability to lipophilic compounds and ATP. In addition it reduces the ability of the cells to accumulate K+. However, the Mpf complex does not dissipate the membrane voltage. The Mpf complex is rapidly disassembled when intracellular ATP concentration is decreased, as measured by a PRD1 adsorption assay.
摘要
IncP 型质粒是广宿主范围的接合质粒。DNA 易位需要 DNA 转移复制功能以及形成交配配对（Mpf）所需的其他因子。Mpf 系统位于细胞膜中，负责将 DNA 从供体转运到受体。Mpf 复合体充当 IncP 特异性噬菌体（如 PRD1）的受体。在本研究中，我们通过噬菌体受体饱和技术对细胞表面的 Mpf 复合体进行定量。电化学测量结果表明，Mpf 复合体增加了细胞包膜对亲脂性化合物和 ATP 的通透性。此外，它降低了细胞积累钾离子的能力。然而，Mpf 复合体并不会消耗膜电压。通过 PRD1 吸附试验测量发现，当细胞内 ATP 浓度降低时，Mpf 复合体迅速解体。
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本文引用的文献
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