Becher G, Winsel K, Beck E, Neubauer G, Stresemann E
FILT Forschungsgesellschaft für Lungen- und Thorax-erkrankungen mbH, Berlin.
Pneumologie. 1997 Apr;51 Suppl 2:456-9.
The detection of mediators from the lower airways still depends on invasive or provoking sampling techniques like bronchoalveolar lavage (BAL) or induced sputum, respectively. Both methods affect the specimen itself. In contrast, the breathing condensate opens the possibility to get native specimens from lower airways during breathing at rest. The breathing condensate was obtained by freezing of exhaled air. The equipment was developed in the FILT Res. Soc. Ltd.. The method is applied for a patent. Leukotriene B4 and Leukotriene C4D4E4F4 were measured in the exhalation of asthmatics, patients with different airway disorders and healthy volunteers. In an additional study the condensate was obtained before and after of a non-specific bronchial challenge test. In asthmatics a close correlation between leukotriene concentration of the condensate and the degree of asthmatic disease according to "International Consensus Report" was found, but no correlation to lung function tests. Within a bronchial challenge test applying histamine the release of leukotrienes was shown to be more sensitive to the challenge test than a lung function test. The results of the study indicate new diagnostic possibilities in lung diseases using the detection of non volatile substances in the exhaled air.
来自下呼吸道介质的检测仍然分别依赖于侵入性或激发性采样技术，如支气管肺泡灌洗（BAL）或诱导痰。这两种方法都会影响样本本身。相比之下，呼吸冷凝物为在静息呼吸时从下呼吸道获取天然样本提供了可能。呼吸冷凝物是通过冷冻呼出的空气获得的。该设备由FILT Res. Soc. Ltd.研发。该方法已申请专利。在哮喘患者、患有不同气道疾病的患者和健康志愿者的呼气中测量了白三烯B4和白三烯C4D4E4F4。在另一项研究中，在非特异性支气管激发试验前后获取冷凝物。在哮喘患者中，根据“国际共识报告”，发现冷凝物中白三烯浓度与哮喘疾病程度密切相关，但与肺功能测试无关。在应用组胺的支气管激发试验中，白三烯的释放显示出比肺功能测试对激发试验更敏感。该研究结果表明，通过检测呼出空气中的非挥发性物质，在肺部疾病诊断方面有新的可能性。
