Rodriguez E, Boudard F, Mallié M, Bastide J M, Bastide M
Laboratoire d'immunologie et parasitologie, Faculté de pharmacie, Montpellier, France.
Can J Microbiol. 1997 Jul;43(7):649-57. doi: 10.1139/m97-092.
The interaction between Aspergillus fumigatus conidia and murine macrophages of various origins was investigated. Cocultures were carried out between A. fumigatus strains and freshly isolated murine pulmonary alveolar macrophages or two murine macrophage cell-lines: murine alveolar cell-line MALU and murine astrocytoma cell-line J774. By measuring the variation of elastolytic activity in the coculture supernatants with two elastin substrates, we demonstrated that either viable or fixed A. fumigatus or C. albicans yeasts or nonspecific particles induced significant macrophage elastolytic activity. The effect of A. fumigatus supernatant or the purified A. fumigatus galactomannan suggested also the possible involvement of this polysaccharide in macrophage-protease gene expression, release, and activity in invasive aspergillosis. The effect of inhibitory compounds demonstrated the potential implication of a macrophagic metalloprotease and a macrophagic cysteine protease. RNA analysis allowed us to demonstrate the induction of expression of two macrophagic protease genes in stimulated macrophages. Two distinctive mechanisms appeared to be implicated in macrophage protease induction: nonspecific phagocytosis in the earliest times of the coculture and (or) specific galactomannan recognition after its gradual release by the mycelium.
研究了烟曲霉分生孢子与各种来源的小鼠巨噬细胞之间的相互作用。在烟曲霉菌株与新鲜分离的小鼠肺泡巨噬细胞或两种小鼠巨噬细胞系（小鼠肺泡细胞系MALU和小鼠星形细胞瘤细胞系J774）之间进行了共培养。通过用两种弹性蛋白底物测量共培养上清液中弹性蛋白酶活性的变化，我们证明了活的或固定的烟曲霉或白色念珠菌酵母或非特异性颗粒均可诱导显著的巨噬细胞弹性蛋白酶活性。烟曲霉上清液或纯化的烟曲霉半乳甘露聚糖的作用也表明该多糖可能参与侵袭性曲霉病中巨噬细胞蛋白酶基因的表达、释放及活性。抑制性化合物的作用证明了巨噬细胞金属蛋白酶和巨噬细胞半胱氨酸蛋白酶的潜在影响。RNA分析使我们能够证明在受刺激的巨噬细胞中两种巨噬细胞蛋白酶基因的表达被诱导。两种不同的机制似乎与巨噬细胞蛋白酶的诱导有关：共培养最早阶段的非特异性吞噬作用和（或）菌丝体逐渐释放半乳甘露聚糖后的特异性半乳甘露聚糖识别。
