Sugase M, Matsukura T
Department of Obstetrics and Gynecology, Nagano Red Cross Hospital, Japan.
Int J Cancer. 1997 Jul 29;72(3):412-5. doi: 10.1002/(sici)1097-0215(19970729)72:3<412::aid-ijc7>3.0.co;2-s.
To clarify the pathogenic relationships between human papillomavirus (HPV) and vaginal intraepithelial neoplasia (VAIN), we examined 71 vaginal biopsy specimens by histopathology and immunohistochemistry and analyzed the presence of HPV DNA by blot hybridization at Tm - 40 degrees C using an HPV 58 probe (PBM-58 method). We found 27 cases of VAIN in patients with previous hysterectomy or antecedent or concomitant cervical intraepithelial neoplasia (CIN) and 44 cases of VAIN in patients without any abnormal findings on the cervix and the vulva. Histopathologically, 53 of 71 cases were graded as VAIN I and 15 and 3 cases were VAIN II and III, respectively, while 59 cases showed positivity for HPV capsid antigen by immunohistochemistry. Using the PBM-58 method, all 71 VAIN cases harbored a single HPV type at more than 1,000 viral copies per cell. We identified 15 different types (HPV 16, 18, 30, 31, 35, 40, 42, 43, 51, 52, 53, 54, 56, 58 and 66). Furthermore, we molecularly cloned 7 novel prototypes (HPV 59, 61, 62, 64, 67, 69 and 71) from VAIN I. Our results are strongly indicative that HPVs are etiologic agents of VAIN, like in the case of CIN. The distinct manifestations of HPV infection in the vagina are discussed in comparison with those in the cervix.
为了阐明人乳头瘤病毒（HPV）与阴道上皮内瘤变（VAIN）之间的致病关系，我们通过组织病理学和免疫组织化学检查了71份阴道活检标本，并使用HPV 58探针在Tm - 40℃下通过印迹杂交分析HPV DNA的存在情况（PBM - 58方法）。我们发现27例VAIN患者既往有子宫切除术史或有先行或同时存在的宫颈上皮内瘤变（CIN），44例VAIN患者宫颈和外阴无任何异常发现。组织病理学上，71例中有53例被分级为VAIN I，15例和3例分别为VAIN II和III，而59例通过免疫组织化学显示HPV衣壳抗原呈阳性。使用PBM - 58方法，所有71例VAIN病例均携带单一HPV型别，每个细胞中病毒拷贝数超过1000个。我们鉴定出15种不同类型（HPV 16、18、30、31、35、40、42、43、51、52、53、54、56、58和66）。此外，我们从VAIN I中分子克隆出7种新型原型（HPV 59、61、62、64、67、69和71）。我们的结果有力地表明，HPV是VAIN的病原体，就像在CIN的情况一样。并将阴道中HPV感染的不同表现与宫颈中的表现进行了比较讨论。
