Madison L D, Bergstrom-Porter B, Torres A R, Shelton E
J Cell Biol. 1979 Sep;82(3):783-97. doi: 10.1083/jcb.82.3.783.
The mesothelial cells of the mouse omentum provide an in vivo model for the study of the mobilization of labile microvilli on the cell surface. These mesothelial cells are sparsely covered with microvilli and large pits 150--400 nm in diameter, termed vesiculated pits. On the unstimulated cell, the microvilli average 44/100 microns2 and pits, 30/100 microns 2 of surface and they are rapidly induced to increase in number by the intraperitoneal injection of isologous mouse serum. After 2 min, microvilli increase threefold, continue to sevenfold at 30 min, and decrease to fourfold at 90 min. Vesiculated pits increased with similar kinetics. Bovine serum albumin and gamma globulin also stimulate the microvilli and pits to form, but the response is a slow, gradual rise to five- or sixfold the normal value at 90 min. Evidence indicates that multiple factors, possibly including insulin and immunoglobulins, are involved in the effect of serum. The close physical and temporal relationship between microvilli and pits suggests that a correlation exists in their mobilization by the cell and it is hypothesized that microvilli function in the regulation of the cortical microfilament network in effecting this mobilization.
小鼠网膜的间皮细胞为研究细胞表面不稳定微绒毛的动员提供了一个体内模型。这些间皮细胞稀疏地覆盖着微绒毛和直径为150 - 400纳米的大坑，称为泡状坑。在未受刺激的细胞上，微绒毛平均每100平方微米有44个，坑平均每100平方微米有30个，通过腹腔注射同种小鼠血清可迅速诱导它们数量增加。2分钟后，微绒毛增加三倍，30分钟时增加到七倍，90分钟时降至四倍。泡状坑以类似的动力学增加。牛血清白蛋白和γ球蛋白也刺激微绒毛和坑的形成，但反应是缓慢逐渐上升，在90分钟时达到正常值的五到六倍。有证据表明，多种因素，可能包括胰岛素和免疫球蛋白，参与了血清的作用。微绒毛和坑之间密切的物理和时间关系表明它们在细胞动员过程中存在相关性，并且推测微绒毛在调节皮质微丝网络以实现这种动员中发挥作用。
