Rumsby G, Weir T, Samuell C T
Department of Chemical Pathology, UCL Hospitals, London, UK.
Ann Clin Biochem. 1997 Jul;34 ( Pt 4):400-4. doi: 10.1177/000456329703400411.
We have developed a sensitive assay for the measurement of alanine:glyoxylate aminotransferase (EC 2.6.1.44) activity in human liver. The assay is partly automated, and takes into consideration the sensitivity of the reaction to pH and to glyoxylate concentration. It is less subject to interference from other enzymes utilizing glyoxylate and to chemical interference from glyoxylate itself and can therefore be used without correction for cross-over by glutamate:glyoxylate aminotranferase (EC 2.6.1.4). The assay allows clear discrimination between normal and affected livers and is sufficiently sensitive to measure enzyme activity in fetal liver samples. Enzyme activity ranged from 17.9 to 38.5 mumol/h/mg protein in control livers (n = 9) and 0.8 to 9.5 mumol/h/mg protein in 30 of 39 hyperoxaluric patients studied. Normal alanine:glyoxylate aminotransferase activity (from 22.8 to 45.5 mumol/h/mg protein) allowed exclusion of primary hyperoxaluria type 1 in the other nine hyperoxaluric patients.
我们开发了一种灵敏的检测方法，用于测定人肝脏中丙氨酸：乙醛酸转氨酶（EC 2.6.1.44）的活性。该检测方法部分自动化，并考虑到了反应对pH值和乙醛酸浓度的敏感性。它较少受到利用乙醛酸的其他酶的干扰，也较少受到乙醛酸本身的化学干扰，因此无需对谷氨酸：乙醛酸转氨酶（EC 2.6.1.4）的交叉反应进行校正即可使用。该检测方法能够清晰地区分正常肝脏和病变肝脏，并且灵敏度足以测量胎儿肝脏样本中的酶活性。在对照肝脏（n = 9）中，酶活性范围为17.9至38.5 μmol/h/mg蛋白质，在所研究的39例高草酸尿症患者中的30例中，酶活性范围为0.8至9.5 μmol/h/mg蛋白质。正常的丙氨酸：乙醛酸转氨酶活性（22.8至45.5 μmol/h/mg蛋白质）可排除其他9例高草酸尿症患者的1型原发性高草酸尿症。
