Hatakeyama S, Gao Y H, Ohara-Nemoto Y, Kataoka H, Satoh M
Department of Oral Pathology, School of Dentistry, Iwate Medical University, Japan.
Biochem Mol Biol Int. 1997 Jul;42(3):497-505. doi: 10.1080/15216549700202901.
Bone morphogenetic proteins (BMPs) are crucial factors of osteogenesis. We investigated the expressions of BMP subtypes in human salivary adenocarcinoma cell line (HSG-S8), tongue squamous cell (HSC-4) and gingival squamous cell (Ca9-22) carcinoma cell lines, gastric poorly differentiated adenocarcinoma cell (MNK45) and signet ring cell (KATOIII) carcinoma cell lines, rectal adenocarcinoma (RCM-1, RCM-2, and RCM-3), and thyroid (8505C) and bladder (T24) carcinoma cell lines by reverse transcription-polymerase chain reaction (RT-PCR). RT-PCR disclosed that BMP-1 was expressed in all cell lines examined, and BMP-2 was amplified in almost all cells except MKN45. Two squamous cell carcinomas, HSC-4 and Ca9-22, and KATOIII expressed only BMP-1 and BMP-2. MKN45 did not express BMP-2, but expressed BMP-7 and weakly BMP-4 and BMP-5. In addition to the expression BMP-7, and HSG-S8 expressed BMP-6. These findings indicated that the neoplastic epithelial cells possessed a rather great potency to express BMP mRNAs. On the other hand, among these carcinoma cells, HSG-S8 solely induced bone in nude mouse tumors, and HSC-4 and KATOIII contained many calcified masses in tumors while the rest did not induce either.
骨形态发生蛋白（BMPs）是成骨的关键因子。我们通过逆转录聚合酶链反应（RT-PCR）研究了BMP亚型在人涎腺癌细胞系（HSG-S8）、舌鳞状细胞（HSC-4）和牙龈鳞状细胞（Ca9-22）癌细胞系、胃低分化腺癌细胞（MNK45）和印戒细胞（KATOIII）癌细胞系、直肠腺癌（RCM-1、RCM-2和RCM-3）以及甲状腺（8505C）和膀胱（T24）癌细胞系中的表达情况。RT-PCR结果显示，BMP-1在所有检测的细胞系中均有表达，BMP-2在除MKN45外的几乎所有细胞中均能扩增出来。两种鳞状细胞癌，即HSC-4和Ca9-22，以及KATOIII仅表达BMP-1和BMP-2。MKN45不表达BMP-2，但表达BMP-7，且微弱表达BMP-4和BMP-5。除了表达BMP-7外，HSG-S8还表达BMP-6。这些发现表明肿瘤上皮细胞具有相当强的表达BMP mRNA的能力。另一方面，在这些癌细胞中，只有HSG-S8在裸鼠肿瘤中诱导形成了骨，HSC-4和KATOIII的肿瘤中有许多钙化团块，而其余细胞系则未诱导形成骨或钙化团块。
