Hatakeyama S, Ohara-Nemoto Y, Kyakumoto S, Satoh M
Department of Oral Pathology, School of Dentistry, Iwate Medical University, Morioka, Japan.
Biochem Biophys Res Commun. 1993 Feb 15;190(3):695-701. doi: 10.1006/bbrc.1993.1105.
A subclone termed as HSG-S8 was previously isolated from human salivary adenocarcinoma cell line, HSG, and proliferated in a serum-free culture. When HSG-S8 and parental HSG cells were transplanted into nude mice i.m. or s.c., both cells reproducibly induced adenocarcinoma. Furthermore, injection of HSG-S8 consistently induced formation of cartilage i.m. or bone s.c. in tumors, but did not parental HSG cells. The cytoplasm of HSG-S8 cells was specifically immunostained by anti-bone morphogenetic protein (BMP)-2 antibody. The conditioned medium of HSG-S8 cells contained a protein of M(r) 18,000 which specifically reacted with anti-BMP-2 protein antibody. Northern blot analysis also revealed that HSG-S8 cells expressed transcripts for BMP-2. On the other hand, parental HSG cells gave a very slightly positive signal only in Northern blot analysis. These results indicate that HSG-S8 cells synthesize and secrete BMP-2 protein, which is probably involved in the formation of cartilage and bone in the tumor tissues in nude mice.
一个名为HSG-S8的亚克隆先前从人唾液腺癌细胞系HSG中分离出来，并在无血清培养中增殖。当将HSG-S8和亲本HSG细胞经肌肉注射或皮下注射到裸鼠体内时，两种细胞均可重复性地诱发腺癌。此外，注射HSG-S8始终会在肿瘤的肌肉部位诱导形成软骨，或在皮下诱导形成骨，但亲本HSG细胞则不会。HSG-S8细胞的细胞质被抗骨形态发生蛋白（BMP）-2抗体特异性免疫染色。HSG-S8细胞的条件培养基含有一种分子量为18,000的蛋白质，该蛋白质与抗BMP-2蛋白抗体发生特异性反应。Northern印迹分析还显示，HSG-S8细胞表达BMP-2的转录本。另一方面，亲本HSG细胞仅在Northern印迹分析中给出非常微弱的阳性信号。这些结果表明，HSG-S8细胞合成并分泌BMP-2蛋白，这可能与裸鼠肿瘤组织中软骨和骨的形成有关。
