Ogose A, Motoyama T, Hotta T, Watanabe H
Department of Pathology, Niigata University School of Medicine, Japan.
Pathol Int. 1996 Jan;46(1):9-14. doi: 10.1111/j.1440-1827.1996.tb03527.x.
In addition to osteosarcomas some epithelial tumors also show bone induction, which is supposedly due to bone morphogenetic proteins (BMPs). However, the types of BMPs expressed in different kinds of tumors have not been studied extensively. In this study five human osteosarcoma and six human carcinoma cell lines were examined to investigate the bone induction and BMP mRNA expression. The cultured cells were transplanted in the subcutaneous tissue of athymic nude mice with or without diffusion chambers. Reverse transcription-polymerase chain reaction (RT-PCR) was used for the detection of BMP mRNAs. All osteosarcoma cell lines expressing all BMPs (BMP-1 to 7) examined, successfully formed bone or osteoid tissue irrespective of the use of a diffusion chamber. Bone formation was induced by diffusion chamber in an osteosarcoma cell line OST and a gastric adenocarcinoma cell line MKN45. OST cells lacked BMP-3 and 7, and MKN45 cells lacked BMP-3, 5 and 6. Our data suggest that BMP-3, 5, 6 and 7 are not always essential for bone induction in tumor cells.
除骨肉瘤外，一些上皮性肿瘤也表现出骨诱导现象，推测这是由骨形态发生蛋白（BMPs）引起的。然而，不同类型肿瘤中表达的BMPs种类尚未得到广泛研究。在本研究中，检测了5种人骨肉瘤细胞系和6种人癌细胞系，以研究骨诱导和BMP mRNA表达。将培养的细胞移植到无胸腺裸鼠的皮下组织中，有无扩散室均可。采用逆转录-聚合酶链反应（RT-PCR）检测BMP mRNAs。所有检测的骨肉瘤细胞系均表达所有BMPs（BMP-1至7），无论是否使用扩散室，均成功形成骨或类骨组织。扩散室在骨肉瘤细胞系OST和胃腺癌细胞系MKN45中诱导了骨形成。OST细胞缺乏BMP-3和7，MKN45细胞缺乏BMP-3、5和6。我们的数据表明，BMP-3、5、6和7并非肿瘤细胞骨诱导所必需。
